FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457369
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG29500.6449934298126896No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25320.5536011404358406No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23180.5068117865443439No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT17190.3758453240162757No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15680.3428304060834906No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT13950.3050053676571871No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCGCTTACAGTTAATGAAAAA13100.28642081120495705No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11320.24750256357558123No Hit
GTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA11280.2466279962131233No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10770.23547726234178532No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9390.2053046883369883No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9340.20421147913391594No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8880.19415395446565029No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8820.19284210342196345No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACATAACCT8810.192623461581349No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8790.19218617790012002No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8650.18912519213151743No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8360.18278457875369777No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA8320.18191001139123988No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8210.17950495114448073No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATCCTCTGACATACT8120.1775371745789505No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT8010.17513211433219128No Hit
GAACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGG7960.17403890512911893No Hit
TGTCTGTTCCGATCAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCT7910.17294569592604658No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGGAGTTTTCGAAAAC7850.17163384488235978No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTCAT7680.16791693359191373No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTC7370.16113903653286515No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC7350.1607017528516362No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7210.15764076708303362No Hit
GGTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT7070.154579781314431No Hit
ATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT7040.1539238557925876No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6990.15283064658951523No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6980.15261200474890077No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6890.15064422818337053No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6870.1502069445021416No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6770.14802052609599686No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6760.14780188425538243No Hit
CAATAGGTCTATTTACAGGGAGTTTTCGAAAACATTGCTTGATTTTTAGT6420.1403680616744904No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6410.14014941983387594No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCGCT6300.13774435958711673No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT6200.13555794118097203No Hit
ATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAA6100.13337152277482733No Hit
GTTTGTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6050.13227831357175499No Hit
ATCCTAACCTAACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT6040.1320596717311405No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5990.13096646252806815No Hit
GTATACATGCATATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAA5940.1298732533249958No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5910.12921732780315237No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5870.1283427604406945No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5860.12812411860008002No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT5860.12812411860008002No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5830.12746819307823662No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5780.12637498387516427No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5640.12331399810656166No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5630.12309535626594718No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTCATTTCAT5600.12243943074410378No Hit
GTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG5540.12112757970041695No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAA5390.11784795209119989No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5380.11762931025058541No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA5270.11522425000382623No Hit
GCAGATGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG5240.11456832448198283No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5210.1139123989601394No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5210.1139123989601394No Hit
CTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCTTG5200.11369375711952492No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAA5150.11260054791645258No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5150.11260054791645258No Hit
CGCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4990.10910227846662103No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAACCCTAATAAA4820.10538536717617504No Hit
GTATAAAGAGTTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4780.10451079981371714No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4770.10429215797310269No Hit
ATTACATATCCCATGAAATGAAGGGAGTAGCCCCAACGTTTGGTTTTATT4750.10385487429187375No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT4720.10319894877003032No Hit
CCTCTGACATACTTTCCAATCAATAGGTCTATTTACAGGGAGTTTTCGAA4720.10319894877003032No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4710.10298030692941584No Hit
ATTTCATATCCCATGAAATGAAGGGAGTAGCCCCAACGTTTGGTTTTATT4670.10210573956695797No Hit
GTCTTATATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT4640.10144981404511455No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4620.10101253036388562No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4610.10079388852327115No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4590.1003566048420422No Hit
CCTATTACATAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACA4580.10013796300142773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)