FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238414
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAACGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG7760.3254842416972158No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7750.3250648032414204No Hit
ATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA7510.31499828030233123No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6500.2726349962669977No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6270.262987911783704No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGG5840.24495205818450258No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5680.23824104289177647No Hit
GGATATTATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG5640.23656328906859497No Hit
GTGTAAAAGGAGCGGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT5360.22481901230632428No Hit
GTGTGTGGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA5330.22356069693893815No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5070.21265529708825825No Hit
CCGCCTCTGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4820.2021693356933737No Hit
GTATACATGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAA4780.20049158187019217No Hit
CCACCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4720.19797495113541988No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTGATAATATCCTCTTTC4610.1933611281216707No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4600.19294168966587533No Hit
ACCACACACAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA4510.1891667435637169No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGAAAACTTCCTATCA4480.18790842819633075No Hit
CATGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGC4440.18623067437314922No Hit
ATTCTTTGACATACTTTCCAATCAATTGGTCTATTGATAGGAAGTTTTCG4360.1828751667267862No Hit
CCGCTACTGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4310.18077797444780927No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4190.1757447129782647No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4170.17490583606667393No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTCTGTATGCATGTAT4160.17448639761087856No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA3980.16693650540656169No Hit
ACATACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG3970.16651706695076632No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGTACGACGGAGT3860.1619032439370171No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3810.15980605165804024No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCACAG3750.15728942092326795No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3730.15645054401167716No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3710.1556116671000864No Hit
TGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGCGG3700.15519222864429102No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3650.1530950363653141No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3620.15183672099792797No Hit
ATTGTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA3450.1447062672494065No Hit
ACATAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG3390.1421896365146342No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3380.1417701980588388No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3340.1400924442356573No Hit
GCTTACTACTAATTCCCTGGATGCCGGATCTTCCAAATTACTTCCCACCC3290.1379952519566804No Hit
ATCCTAACATGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3290.1379952519566804No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3230.1354786212219081No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG3200.13422030585452197No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3160.13254255203134044No Hit
GTGAAGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGCAAA3150.13212311357554507No Hit
CTATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGT3130.1312842366639543No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3090.12960648284077278No Hit
TGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCG3080.12918704438497738No Hit
CATACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG3040.12750929056179586No Hit
GGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCCTTG2920.12247602909225129No Hit
GCCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGGATAATAAGGTTTA2890.12121771372486514No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG2890.12121771372486514No Hit
GCATTAATGCCTCTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2890.12121771372486514No Hit
CACCTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAATTTCAT2880.12079827526906978No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2860.11995939835747901No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC2850.11953995990168363No Hit
TATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAAAA2850.11953995990168363No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAATGCCCTG2850.11953995990168363No Hit
AATTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAGGTGTATA2780.11660389071111595No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2760.1157650137995252No Hit
GCACTAAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGAATTTGGCCAC2750.1153455753437298No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCCGGATCTTCC2740.11492613688793443No Hit
TCCTAATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGA2720.11408725997634367No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT2690.11282894460895752No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA2670.11199006769736677No Hit
GGCCCATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG2650.111151190785776No Hit
CATTAATGCTGGGTAACCACATTGTGTAAAAGGAGCGGCAAAGCCCAAAA2590.10863456005100372No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA2580.10821512159520834No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2560.10737624468361757No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC2530.10611792931623143No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2450.10276242166986838No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2420.10150410630248223No Hit
ATACAGCACTAAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGAATTTG2410.10108466784668685No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2400.10066522939089148No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGA2400.10066522939089148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)