FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238414
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAACGAAAAAAGGAGATTGAAATTAATTATGCCTGCTAGG8730.3661697719093677No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7760.3254842416972158No Hit
ATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA7400.31038445728858205No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGG7250.30409288045165134No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6960.2919291652335853No Hit
GTGTGTGGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA6570.27557106545756543No Hit
GTGTAAAAGGAGCGGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT6080.2550185811235917No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6070.2545991426677963No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5820.24411318127291184No Hit
CCGCCTCTGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA5600.23488553524541345No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5570.2336272198780273No Hit
GTATACATGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAA5550.23278834296643655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5410.22691620458530118No Hit
GGATATTATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG5390.22607732767371044No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTGATAATATCCTCTTTC4900.20552484333973675No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT4650.19503888194485222No Hit
ATTCTTTGACATACTTTCCAATCAATTGGTCTATTGATAGGAAGTTTTCG4430.18581123591735385No Hit
GCTTACTACTAATTCCCTGGATGCCGGATCTTCCAAATTACTTCCCACCC4410.1849723590057631No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTCTGTATGCATGTAT4310.18077797444780927No Hit
CCGCTACTGCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4160.17448639761087856No Hit
CCACCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4090.1715503284203109No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTTCCTTGCCACAG4070.17071145150872014No Hit
CATGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGC3930.1648393131275848No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3890.16316155930440326No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3890.16316155930440326No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3860.1619032439370171No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGAAAACTTCCTATCA3820.1602254901138356No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3760.15770885937906332No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3720.1560311055558818No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTTCCAATTACATATCCCATGAA3680.15435335173270026No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3670.1539339132769049No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3500.1468034595283834No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3410.14302851342622497No Hit
GTGAAGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGCAAA3340.1400924442356573No Hit
TGCATACAGAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGCGG3300.13841469041247578No Hit
GCCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGGATAATAAGGTTTA3280.13757581350088502No Hit
CTATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGT3260.13673693658929426No Hit
ACCACACACAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3220.13505918276611273No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA3190.1338008673987266No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3190.1338008673987266No Hit
ACATAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG3170.1329619904871358No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGTACGACGGAGT3170.1329619904871358No Hit
CATTAATGCTGGGTAACCACATTGTGTAAAAGGAGCGGCAAAGCCCAAAA3120.13086479820815894No Hit
ATCCTAACATGACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3070.12876760592918202No Hit
GCATTAATGCCTCTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3000.12583153673861436No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG2960.12415378291543282No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTGTATGTTCCGG2920.12247602909225129No Hit
GTATCCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCT2900.12163715218066053No Hit
ACATACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG2900.12163715218066053No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG2870.12037883681327438No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC2840.11912052144588824No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCCGGATCTTCC2800.11744276762270672No Hit
ATTGTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA2770.11618445225532058No Hit
TGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCG2760.1157650137995252No Hit
GGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCCTTG2740.11492613688793443No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG2730.11450669843213906No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2720.11408725997634367No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2690.11282894460895752No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACA2690.11282894460895752No Hit
TATCAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAAAA2670.11199006769736677No Hit
TCCTAATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGA2670.11199006769736677No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2660.11157062924157138No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2660.11157062924157138No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC2650.111151190785776No Hit
GTATACACCTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAAT2640.11073175232998063No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2620.10989287541838987No Hit
CACCTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAATTTCAT2550.1069568062278222No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCC2540.1065373677720268No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA2500.10485961394884528No Hit
CCTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAATTTCATGG2450.10276242166986838No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2450.10276242166986838No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA2430.10192354475827763No Hit
CATACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG2390.10024579093509609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)