FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666124
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA48900.7340975554101038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46450.6973176165398635No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGA31040.465979307156025No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCAACCGTG29660.4452624436291141No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT29130.43730596705718455No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT26470.39737346199806645No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG24860.373203787883337No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC24240.3638962115161742No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC23820.3575910791384187No Hit
CATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCAT23800.3572908347394779No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTG23150.34753289177390395No Hit
CAATAGGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGT22340.33537299361680406No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT21620.3245641952549375No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC21230.3187094294755931No Hit
ATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGTACAATATGTT21040.3158571076856561No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATT20880.31345515249413025No Hit
TAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTAA19890.2985930547465637No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT19830.2976923215497415No Hit
GGTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTG17800.2672175150572566No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC17090.2565588388948604No Hit
AACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAAT15870.2382439305594754No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA15740.23629234196636062No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG15700.23569185316847915No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG15600.23419063117377545No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT15000.22518329920555333No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGG14970.22473293260714222No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG14710.22082975542091263No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC14680.22037938882250152No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTAAT14600.21917841122673856No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA14460.21707670043415342No Hit
GGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGTACAAT14360.2155754784394497No Hit
GTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATTCAAGCTAAGCAA14140.2122727900511016No Hit
ATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATTCAAGCTAAGC14070.21122193465480898No Hit
CTAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTA14000.21017107925851644No Hit
ATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAAT13970.20972071266010534No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTTCGGACGGAAACTGC13620.2044664356786424No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATT13550.20341558028234985No Hit
TTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAAAC13220.19846154769982768No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG13190.19801118110141658No Hit
TGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCTT13140.1972605701040647No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACTCAAA12630.1896043379310759No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA12540.18825323813584258No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12400.1861515273432574No Hit
GTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12280.18435006094961298No Hit
GATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCT12160.18254859455596856No Hit
GGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTA12080.1813476169602056No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGTAAGATTCCTATGGGAG11840.17774468417291675No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCATG11810.17729431757450564No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAATTAAGGGAA11710.17579309557980194No Hit
TACATATCCCATGAAATTAAGGGAATAGCCCCATCGCTTGGTTTTATTAG11610.1742918735850983No Hit
CAATTACATATCCCATGAAATTAAGGGAATAGCCCCATCGCTTGGTTTTA11180.16783661900787242No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACT11180.16783661900787242No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA11170.16768649680840203No Hit
GCCCAAAAGACCCACAATTCTTTGACACACTTTCCAATCAATAGGCCTAT11160.16753637460893167No Hit
GTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGA10960.1645339306195243No Hit
GGGCTATTCCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCT10810.16228209762746876No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTAT10750.16138136443064655No Hit
GGTCTTGCATGGTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGAC10450.15687769844653549No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT10370.15567672085077253No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG10240.15372513225765774No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACATGAGGGA10230.15357501005818736No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTG10220.153424887858717No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10210.15327476565924664No Hit
GAATAGCCCCATCGCTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA10180.15282439906083553No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGGCGAACCACTGAACA10140.15222391026295404No Hit
AATTAAGGGAATAGCCCCATCGCTTGGTTTTATTAGGGTTCAAATGTATA9920.1489212218746059No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA9800.14711975548096148No Hit
GAATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA9710.14576865568572817No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACC9600.14411731149155413No Hit
TACCTTGGTAGTCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG9590.14396718929208374No Hit
GTGGGGGAAAGCCCGGCGAACCACTGAACAAATGGCGCTAGTAAACTGAG9590.14396718929208374No Hit
ATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAATAGG9590.14396718929208374No Hit
GTTTGATTTTGAGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCA9560.14351682269367264No Hit
GTATGCATGTATTCAAGCTAAGCAAGCTTTCACTTTTTCGTCCACTTACA9530.14306645609526153No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC9530.14306645609526153No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA9520.14291633389579117No Hit
CATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGC9400.14111486750214675No Hit
TTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCT9400.14111486750214675No Hit
CAAGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGT9390.1409647453026764No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA9280.13931340110850232No Hit
CCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAGTG9120.1369114459169764No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGCGCC9110.13676132371750604No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8970.13465961292492087No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGGCGAACCACTGAA8950.13435936852598016No Hit
GTTTACTAGCGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCCACTGTT8900.1336087575286283No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG8810.13225765773339498No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACCGCCTGTAACACG8780.13180729113498388No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC8560.12850460274663578No Hit
CCTGTAAATAGGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCT8510.12775399174928392No Hit
CCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCTTAGCTTGAATACATGCA8360.1255021587572284No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGC8290.1244513033609358No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG8160.122499714767821No Hit
GAACAAATGGCGCTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC8060.12099849277311732No Hit
GCTTGAATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGT7990.11994763737682473No Hit
TCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT7970.119647392977884No Hit
AGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAA7970.119647392977884No Hit
AATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTAAT7950.11934714857894325No Hit
CCTTACCACAAGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAA7920.11889678198053215No Hit
GGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCTTTTGGGCTTT7910.11874665978106179No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT7780.11679507118794699No Hit
GAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAA7710.1157442157916544No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATG7570.11364250499906925No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7270.10913883901495817No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAAGGGAC7190.10793786141919523No Hit
GTCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7180.10778773921972486No Hit
GCTATGCCTCATCTTCTTATTGGTTCTTCTGGACTACCAAGGTATGTTGC7180.10778773921972486No Hit
ATCCTGGGCTTTCGTAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT7120.10688700602290264No Hit
TTACATATCCCATGAAATTAAGGGAATAGCCCCATCGCTTGGTTTTATTA7090.10643663942449154No Hit
GGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTAA7070.1061363950255508No Hit
CAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCTTAGCTTGAA7040.1056860284271397No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC6960.10448505083137673No Hit
CAATTCTTTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTC6960.10448505083137673No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACCGCCTGTAACA6950.10433492863190638No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6920.10388456203349528No Hit
CTTTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAA6910.1037344398340249No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAAGGGACTCAAGATG6860.10298382883667306No Hit
AAATAGGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCTTTTGG6820.10238334003879158No Hit
CATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGG6820.10238334003879158No Hit
GAGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATC6800.10208309563985085No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCAT6730.10103224024355827No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTG6710.1007319958446175No Hit
CCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTGATTT6710.1007319958446175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)