FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666124
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA50920.7644222397031183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46250.694315172550456No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCAACCGTG32510.48804727047816926No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT31480.47258468393272124No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGA27190.408182260359933No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT25860.3882160078303739No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG24020.36059352312782605No Hit
CATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCAT23510.3529372909548372No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC23130.34723264737496323No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC23000.3452810587818484No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATT22220.33357152722315964No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC21480.3224624844623524No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTG20850.3130047858957191No Hit
ATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGTACAATATGTT20430.30669965351796363No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT20430.30669965351796363No Hit
CAATAGGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGT20100.30174562093544144No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT19680.29544048855768595No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA18840.282830223802175No Hit
TAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTAA18200.27322240303607137No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC17510.2628639712726159No Hit
GGTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTG16600.24920285112081234No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC16140.24229722994517536No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG15990.24004539695311983No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG15780.23689283076424208No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG15430.23163855378277917No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT15360.2305876983864866No Hit
ATGTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATTCAAGCTAAGC15200.2281857431949607No Hit
AACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAAT14750.2214302442187941No Hit
GTGGATATCCTGCCTTGATGCCTTTGTATGCATGTATTCAAGCTAAGCAA14490.21752706703256453No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACTCAAA13870.20821949066540166No Hit
GGCCTATTTACAGGAAGTTTCCTGAAACATTGTTTGATTTTGAGTACAAT13870.20821949066540166No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTTCGGACGGAAACTGC13870.20821949066540166No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTAAT13780.20686839087016834No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATT13760.2065681464712276No Hit
ATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAAT13160.19756081450300547No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA12840.19275690411995364No Hit
GCCCAAAAGACCCACAATTCTTTGACACACTTTCCAATCAATAGGCCTAT12680.19035494892842775No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG12500.1876527493379611No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGG12470.18720238273955No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA12440.1867520161411389No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTAT12120.1819481057580871No Hit
GTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11870.17819505077132786No Hit
TGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCTT11850.17789480637238714No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA11640.1747422401835094No Hit
TTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAAAC11610.1742918735850983No Hit
CTAATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTA11590.17399162918615751No Hit
GTTCAGATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGA11570.1736913847872168No Hit
GGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGCTATTCCCTTA11330.17008845199992795No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACATATTGTACT11250.168887474404165No Hit
GATATTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCT11220.16843710780575388No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG11110.16678576361157982No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTG10970.16468405281899465No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGTAAGATTCCTATGGGAG10830.1625823420264095No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCATG10470.15717794284547623No Hit
GAACAAATGGCGCTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC10410.15627720964865402No Hit
GGGCTATTCCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCT10270.15417549885606885No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAATTAAGGGAA10260.15402537665659846No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG10240.15372513225765774No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACATGAGGGA10230.15357501005818736No Hit
ATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAATAGG10230.15357501005818736No Hit
TACATATCCCATGAAATTAAGGGAATAGCCCCATCGCTTGGTTTTATTAG10220.153424887858717No Hit
CAATTACATATCCCATGAAATTAAGGGAATAGCCCCATCGCTTGGTTTTA10150.15237403246242442No Hit
GTATGCATGTATTCAAGCTAAGCAAGCTTTCACTTTTTCGTCCACTTACA10110.15177354366454293No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10070.15117305486666147No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC10050.15087281046772072No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACC9990.1499720772708985No Hit
GAATAGCCCCATCGCTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA9910.14877109967513558No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9870.14817061087725408No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGGCGAACCACTGAACA9720.14591877788519855No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA9720.14591877788519855No Hit
GTTTACTAGCGCCATTTGTTCAGTGGTTCGCCGGGCTTTCCCCCACTGTT9400.14111486750214675No Hit
TCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT9390.1409647453026764No Hit
GAATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA9260.13901315670956157No Hit
GGTCTTGCATGGTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGAC9140.13721169031591715No Hit
GTTTGATTTTGAGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCA9110.13676132371750604No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA9100.13661120151803569No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGGCGAACCACTGAA9050.13586059052068383No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGCGCC9010.13526010172280237No Hit
CCTGTAAATAGGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCT8780.13180729113498388No Hit
CCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8770.13165716893551352No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGC8760.13150704673604313No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA8720.13090655793816167No Hit
CAAGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGT8700.13060631353922092No Hit
CATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGC8670.1301559469408098No Hit
GGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCTTTTGGGCTTT8670.1301559469408098No Hit
AATTAAGGGAATAGCCCCATCGCTTGGTTTTATTAGGGTTCAAATGTATA8600.12910509154451724No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8570.12865472494610614No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG8540.12820435834769503No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG8220.12340044796464322No Hit
TTGTTTATACAGAAAAACCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCT8180.12279995916676174No Hit
AAATAGGCCTATTGATTGGAAAGTGTGTCAAAGAATTGTGGGTCTTTTGG8160.122499714767821No Hit
CCTTGTAAGTGGACGAAAAAGTGAAAGCTTGCTTAGCTTGAATACATGCA8150.12234959256835064No Hit
GCTTGAATACATGCATACAAAGGCATCAAGGCAGGATATCCACATTGTGT7970.119647392977884No Hit
TACCTTGGTAGTCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG7940.1191970263794729No Hit
CCTTACCACAAGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAA7920.11889678198053215No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC7840.1176958043847692No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATG7760.11649482678900624No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACCGCCTGTAACACG7660.11499360479430257No Hit
ATCCTGGGCTTTCGTAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT7630.11454323819589146No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGCG7500.11259164960277666No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7480.11229140520383593No Hit
AGAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAA7410.11124054980754335No Hit
GAACATATTGTACTCAAAATCAAACAATGTTTCAGGAAACTTCCTGTAAA7400.11109042760807297No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT7270.10913883901495817No Hit
ATTCTTTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCT7200.1080879836186656No Hit
CAATTCTTTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTC7110.10673688382343229No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTG7100.10658676162396191No Hit
CTTTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTCCTGAA7090.10643663942449154No Hit
GTGGGGGAAAGCCCGGCGAACCACTGAACAAATGGCGCTAGTAAACTGAG7080.10628651722502115No Hit
GCTATGCCTCATCTTCTTATTGGTTCTTCTGGACTACCAAGGTATGTTGC7050.10583615062661005No Hit
AATAAAACCAAGCGATGGGGCTATTCCCTTAATTTCATGGGATATGTAAT7040.1056860284271397No Hit
GTCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7010.1052356618287286No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACCGCCTGTAACA6860.10298382883667306No Hit
CATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGG6830.10253346223826196No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6740.10118236244302864No Hit
GAGTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATC6700.10058187364514716No Hit
ATCCTGCCTTGATGCCTTTGTATGCATGTATTCAAGCTAAGCAAGCTTTC6690.10043175144567677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)