FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580545
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG28660.49367404766211054No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25740.4433764824432215No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18220.3138430268110138No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15470.2664737444987038No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14530.25028206254467783No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13660.23529614414041977No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA13170.22685579929204455No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12840.22117148541456735No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT12400.21359240024459775No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA12350.2127311405661921No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT12130.20894159798120734No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT12110.20859709410984503No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11570.19929548958306417No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT11090.19102739667037008No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10930.18827136569947203No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9280.15984979631208607No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG9250.15933304050504268No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA9040.155715749855739No Hit
TTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA8870.15278746694915984No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8790.15140945146371082No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACCTACAAGGCC8480.14606964145759588No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8470.14589738952191475No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8410.14486387790782798No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATGAGGAAAGGAGGGAGTTTG8310.1431413585510167No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8090.1393518159660319No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATCTCC8060.13883506015898853No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8040.13849055628762627No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7970.13728479273785837No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA7940.13676803693081502No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7770.13383975402423584No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7720.1329784943458302No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7690.13246173853878682No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7590.13073921918197556No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7500.1291889517608454No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT7470.12867219595380203No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA7390.12729418046835303No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7330.12626066885426623No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC7320.1260884169185851No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7310.125916164982904No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAT7290.12557166111154175No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC7260.12505490530449836No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7060.12160986659087582No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6990.12040410304110793No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6980.12023185110542679No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6550.11282501787113833No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT6550.11282501787113833No Hit
GTCTATTTACAGGCAGTTTTCGAAAACAGTGCTTGATTTTGAGTACAATA6510.11213601012841383No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6400.11024123883592142No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6390.11006898690024029No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAGATGTGAAACCACA6330.10903547528615352No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG6280.1081742156077479No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6150.10593494044389325No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6120.10541818463684985No Hit
GTACTCAAAATCAAGCACTGTTTTCGAAAACTGCCTGTAAATAGACCTAT6100.10507368076548759No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6090.10490142882980646No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6030.10386791721571972No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT6010.10352341334435747No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5980.10300665753731407No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG5890.10145639011618394No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5860.10093963430914055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)