FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209919
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8990.42826042425888083No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6400.3048795011409163No Hit
GCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTT5540.2639113181751056No Hit
GCACAGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG4810.22913600007621987No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4460.212462902357576No Hit
GTCTTTAAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA4310.2053172890495858No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT3840.18292770068454975No Hit
CCTTCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3830.18245132646401707No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3760.1791167069202883No Hit
GTCCTGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3590.1710183451712327No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3570.17006559673016736No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3550.16911284828910197No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC3530.16816009984803662No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT3480.1657782287453732No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3290.15672711855525226No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT3270.1557743701141869No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG3140.14958150524726205No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3130.14910513102672937No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3060.14577051148300058No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT3010.14338864038033716No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA3000.14291226615980449No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG2780.1324320333080855No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATAACCCATGAA2770.1319556590875528No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA2750.13100291064648745No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2750.13100291064648745No Hit
GGAACATATTGTACAAAAAATCAAAACATGTTTTAGGAAACTTCCTGTAA2600.12385729733849724No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG2580.12290454889743187No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC2560.1219518004563665No Hit
GTTTACAGGAAGTTTCCTAAAACATGTTTTGATTTTTTGTACAATATGTT2540.12099905201530113No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT2510.1195699293537031No Hit
GTCTTCGAGAAGGTTGATGATATGGCAGAGGCAATATTCTGAGCAGGGCT2490.11861718091263775No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2430.11575893558944164No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT2420.11528256136890896No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA2410.11480618714837629No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2310.11004244494304946No Hit
TGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA2290.10908969650198411No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC2280.10861332228145142No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG2230.106231451178788No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT2230.106231451178788No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT2200.10480232851718997No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA2170.10337320585559191No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG2150.10242045741452656No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA2140.10194408319399387No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC2140.10194408319399387No Hit
CTTTAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATGA2120.10099133475292851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)