FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692074
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG44090.6370706022766351No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39260.5672803775318824No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT35440.5120839678993865No Hit
CTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGTTT30540.44128229062210106No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG24130.3486621372858972No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC22210.3209194392507159No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA22130.3197634934992501No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA21780.31470623083658683No Hit
CTCTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGT18830.2720807312512824No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAATTTG18190.2628331652395553No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT16710.24144816883743644No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16100.2326340824825091No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG15820.2285882723523785No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15670.22642087406837996No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA14830.21428344367798818No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT14370.20763675560705935No Hit
CATTTAAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14250.20590283697986053No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA13410.19376540658946875No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA13270.19174250152440345No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA13200.19073104899187082No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC12490.1804720304476111No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA12140.17541476778494786No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT11990.17324736950094932No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11790.17035750512228462No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC11770.17006851868441814No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC11320.16356632383242253No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAC11230.16226588486202342No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA11080.1600984865780249No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT11070.15995399335909166No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10920.15778659507509313No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT10910.15764210185615987No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10900.15749760863722667No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT10840.15663064932362725No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT10560.15258483919349664No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10220.14767206974976663No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9790.14145886133563754No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCTCCTTTC9770.14116987489777105No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC9690.14001392914630517No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCTAATAAA9650.13943595627057223No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9580.13842450373803958No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCTCCTTTCCTCAC9190.1327892681996434No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9140.1320668021049772No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA9130.13192230888604398No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA9080.1311998427913778No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA9020.13033288347777838No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTTAAATGTATA8940.1291769377263125No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8870.12816548519377988No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8830.1275875123180469No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8740.1262870733476478No Hit
GTCTTTGGGTATACATTTAAATCCTAATAAAACCAAACGTTGGGGCTACT8610.12440866150151575No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8600.12426416828258251No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8560.12368619540684955No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8520.12310822253111661No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT8490.1226747428743169No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8330.12036285137138514No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT8150.11776197343058692No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA8070.11660602767912102No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7990.11545008192765513No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCTAAT7920.11443862939512249No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7910.11429413617618925No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC7770.11227123111112396No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC7690.11111528535965808No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7600.10981484638925895No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT7560.10923687351352603No Hit
CATTTACAAGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7540.10894788707565954No Hit
GTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTA7300.10548004982126188No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7260.10490207694552896No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCT7240.10461309050766247No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG7210.10417961085086276No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7120.10287917188046365No Hit
GCATTAAACCGTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT7100.10259018544259718No Hit
ATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGG7080.1023011990047307No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7010.10128974647219806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)