FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692074
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG45720.6606229969627526No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39140.5655464589046836No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT32920.47567167672821115No Hit
CTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGTTT28560.41267263327332043No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA23500.33955906449310336No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG22680.32771062054057803No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA21750.3142727511797871No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC21410.30935998173605717No Hit
CTCTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGT20370.29433268696700066No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAATTTG17670.2553195178550271No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG16290.23537945364224058No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16090.23248958926357588No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT15180.21934070634065145No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14680.21211604539398965No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13870.2004120946603976No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13660.19737773706279965No Hit
CATTTAAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13360.19304294049480258No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA12950.18711871851853992No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA12890.1862517592049405No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC12600.18206145585587669No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12360.17859361860147902No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT12320.17801564572574607No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA12310.17787115250681287No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC11970.17295838306308284No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA11510.166311694992154No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11080.1600984865780249No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT10580.15287382563136312No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAC10540.15229585275563018No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10390.15012845447163164No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT10360.14969497481483193No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT10340.14940598837696548No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC10100.1459381511225678No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9970.14405973927643576No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT9950.1437707528385693No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCTCCTTTC9930.14348176640070281No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9880.14275930030603665No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9870.1426148070871034No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC9610.1388579833948393No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCTCCTTTCCTCAC9220.13322274785644309No Hit
GTCTTTGGGTATACATTTAAATCCTAATAAAACCAAACGTTGGGGCTACT9150.13221129532391046No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT8890.12845447163164633No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8880.12830997841271308No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAATCCTAATAAA8710.1258535936908481No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8570.1238306886257828No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA8570.1238306886257828No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8530.12325271575004985No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTTAAATGTATA8360.12079633102818485No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8080.11675052089805427No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7960.11501660227085542No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7930.11458312261405572No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA7870.11371616330045631No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7860.11357167008152307No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT7840.11328268364365661No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7640.11039281926499189No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7590.10967035317032572No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7560.10923687351352603No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA7490.10822542098099337No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC7470.10793643454312689No Hit
ATATTTGCCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAG7410.10706947522952748No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7320.10576903625912837No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7320.10576903625912837No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7190.1038906244129963No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7170.10360163797512982No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC7080.1023011990047307No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7040.10172322612899776No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAATTCCCT7030.10157873291006454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6980.10085626681539835No Hit
CTTTAATCCCGAGTGGCAAATTCCCTCCTTTCCTCACATTCATTTACAAG6930.10013380072073218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)