FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422741
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15160.3586120106637397No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14400.34063409983890847No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13020.3079899986043464No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12990.30728034422968203No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA12100.2862272644479717No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10390.2457769650921013No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10090.23868042134545736No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9680.22898181155837732No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9420.2228314736446193No Hit
CTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC8700.20579976865267385No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8510.20130529094646601No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8210.19420874719982212No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8050.1904239238682787No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7960.1882949607442855No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7600.17977910824831278No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7560.17883290241542696No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7480.17694049074965523No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7450.17623083637499085No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7180.1698439470030113No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7080.16747843242079666No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6940.16416671200569616No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6820.16132809450703858No Hit
GCTTTATTCTTCTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6700.158489477008381No Hit
TTCTTCTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTC6420.15186603617818004No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6390.15115638180351565No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6300.14902741867952243No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6290.148790867221301No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6270.14831776430485805No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6260.1480812128466366No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6170.1459522497226434No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5870.13885570597599947No Hit
CTTCTACAGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT5840.13814605160133508No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5780.1367267428520063No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT5730.13554398556089897No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5720.13530743410267754No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5660.13388812535334874No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5650.13365157389512727No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5350.12655503014848335No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5190.12277020681693991No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5170.122297103900497No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5120.12111434660938968No Hit
AGGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAAT5110.1208777951511682No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5080.12016814077650381No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5050.11945848640183943No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACAG5040.11922193494361796No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5040.11922193494361796No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5030.11898538348539649No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5010.11851228056895358No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4980.11780262619428919No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4960.11732952327784625No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG4960.11732952327784625No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4920.11638331744496039No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4880.11543711161207454No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4790.11330814848808136No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4720.1116522882805311No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4670.11046953098942378No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT4660.11023297953120233No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4610.109050222240095No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4610.109050222240095No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCATTTTTGCCTTCTG4570.10810401640720914No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4520.10692125911610183No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4470.1057385018249945No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG4470.1057385018249945No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4470.1057385018249945No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4420.10455574453388718No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4410.10431919307566573No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG4380.10360953870100131No Hit
ATCCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4350.10289988432633693No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4340.10266333286811546No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG4260.10077092120234375No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG4250.10053436974412229No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC4240.10029781828590081No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACAGTACCTTGC4240.10029781828590081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)