FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531500
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG34970.6579492003762936No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA30510.574035747883349No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT26550.49952963311382875No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25550.48071495766698025No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATTAAAGTCTAT23630.4445907808090311No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC20110.3783631232361242No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT18250.34336782690498585No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACACACT18130.34111006585136405No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT17690.3328316086547507No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAACCTTGTAAGTGGAAGA17440.32812793979303856No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC14310.26923800564440264No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATT13800.2596425211665099No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCTGAAGCATTGTTTG13460.2532455315145814No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATTAAAG13280.24985888993414862No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12880.2423330197554092No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA12630.2376293508936971No Hit
CATTTGAACCCTAATAAAACCAAGCGATGGGGTTATTCCCTTAATTTCAT12570.23650047036688618No Hit
CAATAGGCCTATTTACAGGAAGTTTTCTGAAGCATTGTTTGATTTTGAGT12400.2333019755409219No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG12380.23292568203198494No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG11620.21862652869238003No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA11210.21091251175917214No Hit
ATTTACAGGAAGTTTTCTGAAGCATTGTTTGATTTTGAGTACAATGTGTT10830.20376293508936968No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC10760.2024459078080903No Hit
CATTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10570.19887111947318908No Hit
ATCAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG10530.19811853245531516No Hit
GGTTCAGATATTGTTTACACAGAAAAACCTTGTAAGTGGAAGAAAAAGTG10430.19623706491063028No Hit
TAATAAAACCAAGCGATGGGGTTATTCCCTTAATTTCATGGGATATGTAA10230.19247412982126058No Hit
GACCCTTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCC10190.19172154280338666No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9910.18645343367826905No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT9580.18024459078080904No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9550.17968015051740358No Hit
GTGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAAT8780.1651928504233302No Hit
CTTCAAAGACTGTGTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATCA8640.1625587958607714No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTGATAATGTCCTCTTTT8610.16199435559736594No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8590.16161806208842897No Hit
GGCCTATTTACAGGAAGTTTTCTGAAGCATTGTTTGATTTTGAGTACAAT8560.16105362182502353No Hit
GTTTGATTTTGAGTACAATGTGTTCTTGTGGTAAGGTACCCCAACTTCCA8450.15898400752587016No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGATGGGGTTATTCCCTTAAT8070.15183443085606774No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTG7530.14167450611476953No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGTTATT7520.14148635936030104No Hit
GTTCAGATATTGTTTACACAGAAAAACCTTGTAAGTGGAAGAAAAAGTGA7370.13866415804327376No Hit
GGATAATAGACTTTAATGCCTTTATCTAAAGGCAAATATTTGGTAAGGTT7310.13753527751646286No Hit
CCTATTGATAATGTCCTCTTTTAAATGAATGTCAGGAAAAGAGGGAGTTT7290.13715898400752588No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTAT7210.13565380997177798No Hit
GGGTTATTCCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCT7170.13490122295390405No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA7150.13452492944496708No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7130.1341486359360301No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTGATCTCCTCC6990.1315145813734713No Hit
CTAATAAAACCAAGCGATGGGGTTATTCCCTTAATTTCATGGGATATGTA6930.1303857008466604No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT6880.129444967074318No Hit
TTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCTGAAGC6720.1264346190028222No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6640.12492944496707431No Hit
GTACAATGTGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6590.12398871119473188No Hit
TCCTAATACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCAGT6550.12323612417685795No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAATTAAGGGAA6460.12154280338664157No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAA6420.12079021636876763No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACACATTGTACTCAAA6400.12041392285983066No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6370.11984948259642521No Hit
TACATATCCCATGAAATTAAGGGAATAACCCCATCGCTTGGTTTTATTAG6350.11947318908748825No Hit
GTTTTCTGAAGCATTGTTTGATTTTGAGTACAATGTGTTCTTGTGGTAAG6330.11909689557855127No Hit
GAATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6300.11853245531514582No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTACCACAAGAACACATTGTACT6270.11796801505174037No Hit
GAATAACCCCATCGCTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA6270.11796801505174037No Hit
CAAGAACACATTGTACTCAAAATCAAACAATGCTTCAGAAAACTTCCTGT6250.11759172154280338No Hit
GTATGCATGTATTCAAGCTAAACAAGCTTTCACTTTTTCTTCCACTTACA6060.11401693320790217No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT6050.11382878645343367No Hit
GGGTATACATTTGAACCCTAATAAAACCAAGCGATGGGGTTATTCCCTTA6050.11382878645343367No Hit
CATCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTT6050.11382878645343367No Hit
GTATTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTCTCATCAATGTC5960.1121354656632173No Hit
CTCTAAGGCCTCCCGGTACAAAGCTGAGGCGGTGTCGAGGAGATCTCGAA5960.1121354656632173No Hit
TGTCAGTTATGTTAACATTAATATGGGCCTAAAAATCAGACAACTATTGT5950.11194731890874883No Hit
CTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT5920.11138287864534337No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATAGACTTTAATGCC5900.1110065851364064No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGTG5850.11006585136406398No Hit
CCTTTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACACACAG5820.1095014111006585No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC5820.1095014111006585No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCT5790.10893697083725305No Hit
GGGCAATACCATGTGGGCGTTCACGGTGGTTTCCATGCGACGCGCAGAGG5710.10743179680150518No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATTCAAGCTAAAC5700.10724365004703669No Hit
GATATTGTTTACACAGAAAAACCTTGTAAGTGGAAGAAAAAGTGAAAGCT5660.10649106302916275No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTGACATTCATTTAAAAG5660.10649106302916275No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5630.10592662276575728No Hit
ATATTTGCCTTTAGATAAAGGCATTAAAGTCTATTATCCTGAACACGCAG5600.10536218250235183No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG5600.10536218250235183No Hit
ACATCGATGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACC5540.10423330197554093No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA5470.10291627469426153No Hit
CAATTACATATCCCATGAAATTAAGGGAATAACCCCATCGCTTGGTTTTA5450.10253998118532456No Hit
GCCTTAATGCCTTTGTATGCATGTATTCAAGCTAAACAAGCTTTCACTTT5400.10159924741298212No Hit
ATGATTAACTGCGTGTTCAGGATAATAGACTTTAATGCCTTTATCTAAAG5380.10122295390404515No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTGATCT5380.10122295390404515No Hit
GTGTTGGGGTGAGTTAATGAATCTGGCTACCTGGGTGGGAAGTAATTTGG5360.10084666039510819No Hit
AAATCAAACAATGCTTCAGAAAACTTCCTGTAAATAGGCCTATTGATTGG5330.10028222013170272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)