FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408797
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA28790.704261528338026No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC26980.6599852738645342No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21170.5178609432065303No Hit
ATACAATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA20430.4997590491123956No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC16270.39799704988050305No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14860.3635056030254625No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC14800.3620378818826948No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA14710.35983630016854334No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12360.30235055541014244No Hit
GGGTAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT10660.2607651230317248No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10290.2517141759846574No Hit
ATGGCAGAGGCAGTATTCTGAGCAGGGCTGACTGTTCCTGAACTGGAGCC10120.24755563274681566No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9400.2299429790336035No Hit
ACTAACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9280.22700753674806812No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG9140.2235828540816102No Hit
GAGTAGGAGCATTCGGGCCAGGGTTCACCCCTCCCCATGGAAGCTTGTTG8850.21648886855823307No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8810.21551038779638793No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT8680.2123303253203913No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8570.2096395032253172No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8500.20792716189208826No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8270.2023008975114788No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8120.1986315946545596No Hit
GTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACCATTCGTTGACATACT7680.18786830627426326No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7620.18640058513149557No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7520.1839543832268828No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7430.18175280151273124No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7400.1810189409413474No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA7240.17710501789396693No Hit
AATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGA7060.17270185446566388No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT6970.17050027275151236No Hit
CCTTCATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6870.16805407084689955No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6840.16732021027551572No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT6590.1612047055139837No Hit
CTATTACCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT6500.1590031237998322No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC6400.1565569218952194No Hit
ATTCTGAGCAGGGCTGACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6280.15362147960968403No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT6220.15215375846691634No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6190.1514198978955325No Hit
ATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTGGAC6140.1501967969432261No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6120.14970755656230356No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6110.14946293637184224No Hit
TCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTGGACC6110.14946293637184224No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6070.14848445560999712No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG6060.14823983541953586No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6040.1477505950386133No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6000.14677211427676817No Hit
CACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTCGTTTTGTGAG5970.14603825370538434No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5970.14603825370538434No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGTCTGGA5940.14530439313400048No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5930.14505977294353922No Hit
CTCTACCATATCGTCAACCTTCTCGAAGACTGGGGACCCTGTACCGAACA5920.14481515275307794No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACACCCAATCACATATCCCATGAA5900.14432591237215536No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACCATTCG5890.1440812921816941No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5830.1426135710389264No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5820.14236895084846513No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5800.14187971046754258No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5700.13943350856292977No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAATTTCAT5680.1389442681820072No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5670.13869964799154594No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATAT5670.13869964799154594No Hit
CTCCCACTCCCACCTTGTTGGCATCCGGCCAGTTGTCCTTGTAGGGATTG5600.13698730665831696No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5560.13600882589647184No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5520.13503034513462672No Hit
GAGTAGGAGCATTCGGGCCAGGGTTCACCCCTCCCCATGGAGGCTTGTTG5330.1303825615158624No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5320.13013794132540113No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCTGG5250.12842559999217215No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGC5210.12744711923032703No Hit
TTGTAGGGATTGAGGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGC5180.1267132586589432No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT5140.12573477789709805No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5140.12573477789709805No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATC5100.12475629713525295No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA5020.12279933561156271No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4980.12182085484971758No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4970.12157623465925631No Hit
CTGTTAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4930.12059775389741119No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4860.11888541256418222No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC4850.11864079237372094No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT4830.11815155199279839No Hit
GTAGAGGAGACACAAAGGTGCCGCGCATGCGCTGATGGCCTATGGCCAAG4780.11692845104049197No Hit
GATGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA4770.1166838308500307No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4740.11594997027864686No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4700.11497148951680175No Hit
AACCCTATTACCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACAT4680.11448224913587918No Hit
GGGTAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT4670.1142376289454179No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4650.11374838856449533No Hit
GTATGGATCGGTAGAGGAGACACAAAGGTGCCGCGCATGCGCTGATGGCC4630.11325914818357279No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTCATGCC4620.11301452799311149No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4600.11252528761218894No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC4580.11203604723126637No Hit
CCCTAAAAGACCCACCATTCGTTGACATACTTTCCAATCAATGGGCCTGT4510.11032370589803742No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT4500.11007908570757614No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTCATGCC4500.11007908570757614No Hit
ATCACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTCGTTTTGTG4480.10958984532665357No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4480.10958984532665357No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4460.10910060494573101No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4400.10763288380296332No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT4390.10738826361250205No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4390.10738826361250205No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC4390.10738826361250205No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4380.10714364342204077No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4330.10592054246973436No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4310.10543130208881181No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4310.10543130208881181No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4290.10494206170788925No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4270.10445282132696668No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4260.1042082011365054No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4210.10298510018419901No Hit
CTCCCACTCCCACCTTGTTGGCATCCGGCCAGTTGTCCTTGTGGGGATTG4190.10249585980327644No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4170.10200661942235388No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCTTCCATG4160.10176199923189261No Hit
CCCAATCACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTCGTTT4160.10176199923189261No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCTCCATG4140.10127275885097003No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC4130.10102813866050876No Hit
GGGCTGACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCT4110.10053889827958619No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4110.10053889827958619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)