FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408797
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC27350.6690362209116016No Hit
ATACCATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA24250.5932039618686047No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21150.5173717028256078No Hit
ATACAATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA16730.40924957864172196No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15630.3823413576909811No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC15270.373535030834375No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA14150.34613756950271163No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC13330.32607871388488663No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9690.23703696455698062No Hit
GGGTAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9670.23654772417605802No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9630.23556924341421293No Hit
ATGGCAGAGGCAGTATTCTGAGCAGGGCTGACTGTTCCTGAACTGGAGCC8990.219913551224691No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8870.21697810893915562No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8720.2133088060822364No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG8200.20058855617824983No Hit
ACTAACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8150.19936545522594346No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8010.19594077255948553No Hit
CCTTCATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7940.19422843122625655No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7810.1910483687502599No Hit
GAGTAGGAGCATTCGGGCCAGGGTTCACCCCTCCCCATGGAAGCTTGTTG7790.19055912836933736No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT7710.1886021668456471No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7600.18591134475057303No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7030.17196799389428005No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6980.17074489294197365No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6860.1678094506564383No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA6770.16560786894228677No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA6710.16414014779951908No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6650.16267242665675138No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC6650.16267242665675138No Hit
GTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACCATTCGTTGACATACT6530.15973698437121603No Hit
AATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGA6520.15949236418075474No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACACCCAATCACATATCCCATGAA6370.15582306132383555No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6310.15435534018106786No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6170.15093065751460993No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6140.1501967969432261No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6080.14872907580045844No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6070.14848445560999712No Hit
CTATTACCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT6060.14823983541953586No Hit
CTCTACCATATCGTCAACCTTCTCGAAGACTGGGGACCCTGTACCGAACA6040.1477505950386133No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT5950.1455490133244618No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAATTTCAT5900.14432591237215536No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT5860.14334743161031027No Hit
ATTCTGAGCAGGGCTGACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5770.14114584989615872No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5770.14114584989615872No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5760.14090122970569746No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5710.13967812875339106No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5670.13869964799154594No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGTCTGGA5670.13869964799154594No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5620.1374765470392395No Hit
CTCCCACTCCCACCTTGTTGGCATCCGGCCAGTTGTCCTTGTAGGGATTG5580.13649806627739441No Hit
ATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTGGAC5480.1340518643727816No Hit
TCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTGGACC5450.13331800380139777No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGC5440.13307338361093649No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5420.1325841432300139No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5400.13209490284909134No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5350.13087180189678496No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG5330.1303825615158624No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5290.12940408075401727No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5280.129159460563556No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5220.12769173942078832No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATAT5200.12720249903986575No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA5170.12646863846848191No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCTGG5150.12597939808755937No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5090.12451167694479168No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5060.12377781637340783No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTCATGCC5030.12304395580202399No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4980.12182085484971758No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATGGTGGGTCT4980.12182085484971758No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4940.12084237408787246No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4840.11839617218325967No Hit
CACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTCGTTTTGTGAG4790.11717307123095327No Hit
TTGTAGGGATTGAGGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGC4790.11717307123095327No Hit
CTGTTAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4730.11570535008818558No Hit
GGGTAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT4690.11472686932634045No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTCATGCC4680.11448224913587918No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4630.11325914818357279No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4610.11276990780265021No Hit
GTATGGATCGGTAGAGGAGACACAAAGGTGCCGCGCATGCGCTGATGGCC4460.10910060494573101No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC4440.10861136456480845No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC4430.10836674437434717No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT4420.10812212418388589No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACCATTCG4380.10714364342204077No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATC4360.1066544030411182No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4340.10616516266019566No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4320.10567592227927308No Hit
AACCCTATTACCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACAT4320.10567592227927308No Hit
CCCTAAAAGACCCACCATTCGTTGACATACTTTCCAATCAATGGGCCTGT4290.10494206170788925No Hit
CTCCTCTACCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC4270.10445282132696668No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCTTCCATG4270.10445282132696668No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4200.10274047999373771No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT4170.10200661942235388No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA4130.10102813866050876No Hit
GAGTAGGAGCATTCGGGCCAGGGTTCACCCCTCCCCATGGAGGCTTGTTG4090.10004965789866364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)