FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644891
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143322.222391070739086No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT23250.3605260423854574No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG18630.2888860288017665No Hit
ATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT18290.28361382000989316No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG17880.2772561564667517No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT17520.2716738177459447No Hit
CCTATAAATAGACCGATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT16800.2605091403043305No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT15050.23337277152262942No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG13860.21492004075107266No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13710.2125940662840697No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13050.20235977862925672No Hit
TTCTCATACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC12760.1978628946597177No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG12750.19770782969525083No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTAAGGTT11440.17739431935009173No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC10460.16219795283233912No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA10260.15909665354300184No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTA10160.1575460038983332No Hit
CCTGTAAATAGACCGATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT10060.1559953542536646No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT9920.15382444475112847No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9840.15258392503539356No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG9540.14793197610138767No Hit
ATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAAC9480.14700158631458649No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA9270.14374522206078236No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA9050.14033379284251135No Hit
ATCTAAGGGTAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA8830.13692236362424037No Hit
CTTGTATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT8760.13583690887297234No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8580.1330457395125688No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATA8470.1313400249034333No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT8430.13071976504556584No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATAT8320.12901405043643036No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7920.1228114518577558No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7910.12265638689328894No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7890.12234625696435522No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATCAAAC7860.12188106207095463No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT7660.11877976278161737No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA7530.11676391824354812No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT7280.11288729413187655No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT7250.11242209923847596No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6900.10699482548213574No Hit
GAATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA6800.10544417583746711No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6740.10451378605066593No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6630.10280807144153042No Hit
GTGGATACCCAGCGTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG6490.10063716193899434No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6490.10063716193899434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)