FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619909
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA23900.38554045835759765No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT23520.3794105263837112No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCGTGTTCAGG19920.3213374866311023No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT19210.3098841926798933No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT19040.3071418546915757No Hit
AATAAAACCAAGCGATGGGGATACTCCCTTAATTTCATGGGATATGTTAT17180.27713745081939445No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16780.27068489084688235No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT15650.2524564089245357No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT14570.23503449699875303No Hit
CGATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATAACATATCCCATG14030.2263235410358617No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14020.22616222703654892No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATTAAACCCTAT12880.20777243111488944No Hit
CAATAGGCCTATTTACAGGAAGTTTTCTGAAACATTGTTTAATTTTGCGT12430.20051330114581337No Hit
CTTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCTGAAACATTGTTTA12400.20002935914787495No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGC12260.1977709631574957No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCGTTGTATGCATGTATT12120.19551256716711646No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAAACCTTGTAAGTGGACGA11850.19115708918567081No Hit
GGTTAAGATATTGTTTACACAGAAAAACCTTGTAAGTGGACGAAAACGTG11560.18647898320559952No Hit
CCCTTAATTTCATGGGATATGTTATTGGAAGTTGGGGTACCTTACCACAA11550.18631766920628673No Hit
TGTTTACACAGAAAAACCTTGTAAGTGGACGAAAACGTGAAAGCTTGCTT11450.1847045292131587No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11440.18454321521384592No Hit
ATCAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG11210.18083299322965146No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC11150.17986510923377463No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG10630.1714767812695089No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10630.1714767812695089No Hit
CCCTCATAAAACCAAGAGATGGGGATACTCCCTTAATTTCATGGGATATG10530.1698636412763809No Hit
CATGCATACAACGGCATTAAGGCAGGATATCCACATTGTGTAAAAGGGGC10510.16954101327775528No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC10510.16954101327775528No Hit
GTACAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATAACATATCCC10410.16792787328462727No Hit
CCTATTGATAATGTCCTCTTTTAAATGAATGTCAGGAAAAGAGGGAGTTT10060.16228188330867918No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG10040.16195925531005356No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATAATTAACCT9470.15276435734922383No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9450.15244172935059822No Hit
CTAATAAGACCAAGCGATGGGGATACTCCCTTAATTTCATGGGATATGTT9240.1490541353650294No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTGATAATGTCCTCTTTT9010.14534391338083494No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8960.1445373433842709No Hit
ATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATAACATATCCCATGAA8610.13889135340832282No Hit
GTTTTAAAGTAATGATTAACTGCGTGTTCAGGATAATAGGGTTTAATGCC8600.13873003940901005No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATAACATATCCCATG8510.1372782134151948No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8490.13695558541656921No Hit
ATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTG8450.136310329419318No Hit
GTGGATATCCTGCCTTAATGCCGTTGTATGCATGTATTCAAGCTAAGCAA8370.13501981742481559No Hit
GCCCAAAAGACCCACAATTCTTTGACACACTTTCCAATCAATAGGCCTAT8200.13227747943649795No Hit
GACCTGCTGCGAGCAAAACACGCTGCAAGGAGTTCCGCAGTATGGATCGG8200.13227747943649795No Hit
TAATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACACACAG8140.13130959544062112No Hit
CAAGCGATGGGGATACTCCCTTAATTTCATGGGATATGTTATTGGAAGTT8110.1308256534426827No Hit
GCCATGGAAATGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC8090.1305030254440571No Hit
GCATTAAGGCAGGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAA8030.1295351414481803No Hit
ATAGATCCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7930.12792200145505225No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7920.12776068745573949No Hit
TTGACACACTTTCCAATCAATAGGCCTATTTACAGGAAGTTTTCTGAAAC7880.12711543145848828No Hit
CCCTTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTT7810.12598623346329865No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCGTGTTCA7660.12356652347360661No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGC7570.12211469747979138No Hit
GAATACATGCATACAACGGCATTAAGGCAGGATATCCACATTGTGTAAAA7510.12114681348391458No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG7480.12066287148597617No Hit
TTGTTTACACAGAAAAACCTTGTAAGTGGACGAAAACGTGAAAGCTTGCT7410.11953367349078654No Hit
ATATTTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACACACA7350.11856578949490974No Hit
ATGTGGATATCCTGCCTTAATGCCGTTGTATGCATGTATTCAAGCTAAGC7200.11614607950521771No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACACC7200.11614607950521771No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT7190.11598476550590489No Hit
GAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACCATGTGGGCGT7110.11469425351140249No Hit
CTATTATCCTGAACACGCAGTTAATCATTACTTTAAAACTAGGCATTATT6930.11179060152377204No Hit
GGCCTATTTACAGGAAGTTTTCTGAAACATTGTTTAATTTTGCGTACGAT6680.10775775154095199No Hit
GTTTAATTTTGCGTACGATATGTTCTTGTGGTAAGGTACCCCAACTTCCA6660.10743512354232637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA6620.10678986754507516No Hit
GCTTGAATACATGCATACAACGGCATTAAGGCAGGATATCCACATTGTGT6510.10501541355263434No Hit
GTGTCAACAATATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC6440.10388621555744472No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6350.1024343895636295No Hit
ATTAACAACAGTGCCCGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6340.10227307556431671No Hit
CATTGCTGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACCA6330.1021117615650039No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTG6320.10195044756569109No Hit
GAAATGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTCCCGATA6260.10098256356981428No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA6210.10017599357325026No Hit
AAATTAAACAATGTTTCAGAAAACTTCCTGTAAATAGGCCTATTGATTGG6210.10017599357325026No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGTG6200.10001467957393748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)