FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517583
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19140.3697957622255754No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG16420.3172438043753369No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14620.282466773445032No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13270.25638400024730335No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12620.24382562796691543No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG12190.2355177816891204No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT12080.233392518687824No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11670.2254710838648101No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11660.2252778781374195No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11430.2208341464074361No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11310.21851567767874913No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11130.21503797458571863No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGATAGTCCAA11100.21445835740354688No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9590.18528429256756887No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9370.18103376656497605No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8140.15726946209593437No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8070.1559170220042003No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7890.1524393189111698No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG7830.1512800845468263No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7820.15108687881943572No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7810.15089367309204513No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7780.1503140559098734No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7700.14876841009074873No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC7490.14471108981554648No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7430.143551855451203No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7420.1433586497238124No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG6960.1344711862638456No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATT6940.13408477480906444No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6750.13041386598864338No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT6720.1298342488064716No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6520.12597013425865997No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6490.1253905170764882No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6450.12461769416692589No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6390.1234584598025824No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6230.12036716816433307No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6160.11901472807259898No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA5930.11457099634261558No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG5900.11399137916044383No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5900.11399137916044383No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5770.11147970470436626No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCTTTC5710.11032047034002276No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5660.10935444170306985No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5630.10877482452089808No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5630.10877482452089808No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC5530.10684276724699227No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5490.10606994433742993No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5430.10491070997308645No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5420.10471750424569586No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCCTGGTGGAATGTGCTGG5410.10452429851830528No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG5410.10452429851830528No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5370.10375147560874294No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5340.1031718584265712No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAA5330.1029786526991806No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA5310.10259224124439945No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5250.10143300688005595No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5240.10123980115266536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)