FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463548
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTACTAAAACTAAACGTTGGGGATATTCCCTTAACTTCAT29420.6346699802393712No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCT19300.41635386195172885No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19060.4111764046010337No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17340.37407129358771907No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16240.3503412807303667No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA15460.3335145443406076No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14630.31560917100278724No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA14490.31258898754821507No Hit
AGTCTGTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTT13740.29640943332729297No Hit
CAATAGGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGT12950.2793669695479217No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC12680.27354233002838974No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12540.2705221465738176No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA12210.26340314271661186No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATA12070.26038295926203975No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11870.2560684114697938No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11460.2472235884956898No Hit
GAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACA11330.24441913243072994No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCTCTTTTTACC11050.23837876552158568No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG10960.23643721901507503No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT10850.23406421772933977No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT10790.232769853391666No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10690.2306125794955431No Hit
AACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACACAGTCTTT10670.2301811247163185No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10470.22586657692407258No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTAGGAAACTTCCTACAA9720.20968702270315048No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9720.20968702270315048No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9660.2083926583654767No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT9660.2083926583654767No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9510.20515674752129232No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG9460.2040781105732308No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCTAAAAC9030.19480183281990215No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC8740.1885457385211456No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.18789855635230873No Hit
GTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8690.1874671015730841No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8690.1874671015730841No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG8120.17517064036518334No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8110.17495491297557103No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTAGGAAA8000.1725819116898358No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA8000.1725819116898358No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC7550.16287417915728253No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAA7520.16222699698844564No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7490.16157981481960876No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7440.1605011778715473No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7370.15899108614426122No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT7020.1514406275078309No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGATATT7010.1512249001182186No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCAACGTTTAGTTTTA6950.14993053578054485No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6940.14971480839093254No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC6890.14863617144287106No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA6750.14561598798829894No Hit
GGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAAT6740.14540026059868666No Hit
GGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCGATA6730.14518453320907435No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6640.14324298670256372No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6580.14194862236488992No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCCCGAA6580.14194862236488992No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC6570.14173289497527763No Hit
GTTAAGGGAATATCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC6550.14130144019605306No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6460.13935989368954238No Hit
TCACCATACAGCGCTAAGACGACGAGGCAGGACCCCTAGAAGAAGAACTC6460.13935989368954238No Hit
TTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGG6430.13871271152070552No Hit
GCCAATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGT6420.1384969841310932No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATA6360.13720261979341947No Hit
AGTTAAGGGAATATCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6290.13569252806613338No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6220.13418243633884733No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6210.133966708949235No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6200.13375098155962276No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6110.13180943505311207No Hit
GTGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC6090.1313779802738875No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6070.1309465254946629No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTC6030.13008361593621373No Hit
GATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCGATAG5980.12900497898815225No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5810.12533761336474325No Hit
CTGCCAATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCG5750.12404324902706948No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCGCTAAGACG5660.12210170252055882No Hit
CTGTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTTTTG5630.12145452035172194No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5570.12016015601404816No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG5510.1188657916763744No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA5480.11821860950753751No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA5460.11778715472831293No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGT5460.11778715472831293No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5400.11649279039063916No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5380.11606133561141457No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5380.11606133561141457No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5370.11584560822180227No Hit
CTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTG5360.11562988083218997No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACACAAA5310.11455124388412852No Hit
GGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGATATTCCCTTA5270.11368833432567932No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5240.11304115215684245No Hit
GTTTGATTTTGTGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCA5230.11282542476723015No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5200.11217824259839326No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5100.11002096870227031No Hit
ATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT5090.10980524131265802No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCTCTTTTTACCGCTGT5010.10807942219575965No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4980.10743224002692278No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4940.1065693304684736No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4940.1065693304684736No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4930.10635360307886131No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4920.10613787568924901No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4910.10592214829963673No Hit
TCCTAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGTAAGGTA4890.10549069352041211No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAAGTCTTGCCC4840.10441205657235066No Hit
GCACGGGACCTTGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4830.10419632918273836No Hit
AACTAAACGTTGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGAA4820.10398060179312606No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4820.10398060179312606No Hit
ATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCGA4800.10354914701390147No Hit
AACATATTGTACACAAAATCAAACAATGTTTTAGGAAACTTCCTACAAAT4760.1026862374554523No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT4750.10247051006583999No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACACAGTCTTTGA4710.10160760050739082No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4700.10139187311777853No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4670.10074469094894165No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC4660.10052896355932935No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC4660.10052896355932935No Hit
ATATTGTACACAAAATCAAACAATGTTTTAGGAAACTTCCTACAAATAGG4660.10052896355932935No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGC4660.10052896355932935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)