FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463548
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTACTAAAACTAAACGTTGGGGATATTCCCTTAACTTCAT25420.5483790243944532No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCT17910.3863677547956199No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17230.3716982923019838No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA16930.36522647061361496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16740.36112765021098137No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA12980.2800141517167586No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12820.27656251348296185No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT12700.2739737848076143No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12530.2703064191842053No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG12300.26534468922312254No Hit
AGTCTGTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTT11900.2567155936386307No Hit
CTGCCAATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCG11500.24808649805413896No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCTCTTTTTACC11330.24441913243072994No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA11230.242261858534607No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11150.24053603941770865No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATA10930.23579003684623812No Hit
CAATAGGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGT10910.23535858206701357No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA10800.23298558078127832No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10370.2237093030279496No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10350.22327784824872507No Hit
GAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACA10310.2224149386902759No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9730.20990275009276277No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9480.2045095653524554No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT9410.20299947362516935No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8900.1919973767549423No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8860.19113446719649313No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG8740.1885457385211456No Hit
GTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8640.18638846462502265No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8490.18315255378083822No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8430.18185818944316445No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8380.180779552495103No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT8060.17387627602750955No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTAGGAAA7980.1721504569106112No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAA7920.17085609257293743No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTAGGAAACTTCCTACAA7880.16999318301448826No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7760.16740445433914072No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7590.1637370887157317No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7540.16265845176767021No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGATATT7460.16093263265077187No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCTAAAAC7420.1600697230923227No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC7050.1520878096766678No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7010.1512249001182186No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG6920.14928335361170797No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCCCGAA6900.14885189883248337No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG6850.14777326188442189No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC6780.14626317015713583No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC6770.14604744276752352No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6710.14475307842984977No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA6590.14216434975450223No Hit
GGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCGATA6560.14151716758566535No Hit
AACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACACAGTCTTT6520.14065425802721618No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6480.139791348468767No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6430.13871271152070552No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAAGCTCTTTTTACCGCTGT6430.13871271152070552No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6420.1384969841310932No Hit
TTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGG6340.13677116501419487No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6320.13633971023497027No Hit
GTGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC6320.13633971023497027No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG6050.1305150707154383No Hit
CTGTACAACATCTTGAAGCTCTTTTTACCGCTGTTACCAATTTTCTTTTG6030.13008361593621373No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6010.12965216115698913No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCAACGTTTAGTTTTA6000.12943643376737685No Hit
GCCAATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGT5870.12663197770241702No Hit
GGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAAT5840.12598479553358014No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA5800.12512188597513096No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5770.12447470380629407No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA5630.12145452035172194No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGT5510.1188657916763744No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5500.1186500642867621No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5480.11821860950753751No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5480.11821860950753751No Hit
GTTAAGGGAATATCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC5470.11800288211792523No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5400.11649279039063916No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACACAAA5360.11562988083218997No Hit
CTTTCCAATCAATAGGCCTATTTGTAGGAAGTTTCCTAAAACATTGTTTG5300.1143355164945162No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5300.1143355164945162No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5180.11174678781916868No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5170.11153106042955636No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5170.11153106042955636No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5130.1106681508711072No Hit
TCACCATACAGCGCTAAGACGACGAGGCAGGACCCCTAGAAGAAGAACTC5080.10958951392304572No Hit
CTTCAAAGACTGTGTATTTACAGACTGGGAGGAGTTGGGGGAGGAGACTA5050.10894233175420885No Hit
ATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT5050.10894233175420885No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATA5030.10851087697498424No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC5020.10829514958537195No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAAGTCTTGCCC5000.10786369480614737No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5000.10786369480614737No Hit
GGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGATATTCCCTTA4980.10743224002692278No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA4970.10721651263731048No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4940.1065693304684736No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4940.1065693304684736No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4920.10613787568924901No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4860.10484351135157524No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4830.10419632918273836No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCGCTAAGACG4800.10354914701390147No Hit
GCCCAGAAGCCCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTAT4760.1026862374554523No Hit
GTTTGATTTTGTGTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCA4760.1026862374554523No Hit
AGTTAAGGGAATATCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4740.1022547826762277No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4700.10139187311777853No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4700.10139187311777853No Hit
GATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCGATAG4700.10139187311777853No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGG4690.10117614572816623No Hit
CCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG4660.10052896355932935No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTC4640.10009750878010476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)