FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203678
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11670.5729632066300729No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8940.4389281120199531No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT8520.41830732823378075No Hit
CAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGG6420.3152034093029193No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6090.29900136489949825No Hit
GAATTTCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAGAC5450.26757921817771185No Hit
GCACGCTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5440.267088247135184No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5250.25775979732715365No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5180.25432300002945823No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC5170.25383202898693036No Hit
GAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATA4990.24499455022142796No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4970.24401260813637213No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4950.2430306660513163No Hit
CTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA4340.2130814324571137No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4310.21160851932952995No Hit
CCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG4290.21062657724447414No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4090.2008071563939159No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4060.19933424326633215No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3930.1929516197134693No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3850.189023851373246No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGTTTCCTGGAAGTAGAGG3820.18755093824566227No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC3730.18313219886291104No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3590.1762586042675203No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG3550.17429472009740865No Hit
CTCCCAGTACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAG3520.17282180696982494No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC3510.172330835927297No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC3400.16693015445948997No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG3270.16054753090662713No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT3250.15956558882157132No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3170.15563782048134803No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG3110.15269199422618054No Hit
GAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAGAC3110.15269199422618054No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTA3090.15171005214112473No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3040.14925519692848516No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2950.14483645754573396No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2920.14336354441815022No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC2910.1428725733756223No Hit
ATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC2910.1428725733756223No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG2850.13992674712045483No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2810.13796286295034318No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCGGGGGAGGAGATTA2720.133544123567592No Hit
GAAACTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTC2720.133544123567592No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2670.1310892683549524No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG2660.1305982973124245No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2660.1305982973124245No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG2640.1296163552273687No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2590.12716150001472912No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAGATCAGACAACTATT2590.12716150001472912No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2580.1266705289722012No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2580.1266705289722012No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT2550.1251976158446175No Hit
CCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGAAGTATGCCTCA2540.12470664480208958No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA2520.12372470271703376No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2520.12372470271703376No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAGGTCTTGCCT2520.12372470271703376No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2500.12274276063197792No Hit
CGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA2500.12274276063197792No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC2480.1217608185469221No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT2440.11979693437681047No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2440.11979693437681047No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2420.11881499229175463No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA2390.11734207916417089No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA2380.11685110812164297No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG2370.11636013707911508No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG2360.11586916603658717No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCAGGTCTG2360.11586916603658717No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCC2360.11586916603658717No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTGACAGTTAACG2340.11488722395153134No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2270.11145042665383596No Hit
CTCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAG2260.11095945561130804No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTAACC2250.11046848456878014No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG2240.10997751352625222No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2220.1089955714411964No Hit
ACACTTGGGTGCTCCCCCTAGAGAATTGAGAGAAGTCCACCACGAGTCTA2210.10850460039866848No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA2190.10752265831361267No Hit
CACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG2190.10752265831361267No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2180.10703168727108475No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA2170.10654071622855685No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC2160.10604974518602893No Hit
CGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCA2140.1050678031009731No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2140.1050678031009731No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC2120.10408586101591727No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2110.10359488997338938No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT2090.10261294788833356No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCCGGATCTTCC2080.10212197684580564No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2060.10114003476074981No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC2050.1006490637182219No Hit
GACGTAGACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTA2040.10015809267569398No Hit
CCTACCGCTTACAGACCACCAAATACTCAAGAACAGTTTCTCTTCCAAAA2040.10015809267569398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)