FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555132
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38540.6942492956630135No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT20350.3665794801957012No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT16350.2945245455134995No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA14680.26444161028368024No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13020.23453881239056656No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT12820.23093606565645647No Hit
GTCTCTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT12790.23039565364633996No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG11740.211481233292262No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10590.19076543957112904No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10510.18932434087748498No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10230.18428049544973088No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9850.17743527665492173No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9380.16896882182976303No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9180.16536607509565293No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9020.16248387770836484No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8790.15834071896413826No Hit
CCCAAAGACAGAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG8740.15744003228061074No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC8680.1563592082603777No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8550.15401742288320616No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8210.14789275343521902No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8080.14555096805804746No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7690.1385256119265328No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA7570.13636396388606672No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7530.13564341453924472No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT7480.1347427278557172No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7050.1269968223773805No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6980.12573586102044199No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6740.12141256493950989No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT6690.12051187825598236No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6580.11853036755222182No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6530.1176296808686943No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6520.11744954353198878No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6480.11672899418516677No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6430.11582830750163926No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6430.11582830750163926No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6260.11276597277764568No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6180.11132487408400163No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6140.11060432473717964No Hit
GGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT5960.10736185267648055No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5810.10465979262589799No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5800.10447965528919247No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5750.10357896860566496No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5730.10321869393225395No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5690.10249814458543192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)