FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591747
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG38740.6546716755640501No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT27210.4598248913809449No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25180.42551969000265316No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT21350.3607960834613441No Hit
TATCCATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA19330.3266598732228469No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCCGG17660.29843835287715864No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA17340.29303063640373334No Hit
GTGTACAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATC15960.2697098591120868No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT15430.26075332870297613No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG15040.25416267425098904No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG14160.23929145394906948No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCACAG12270.20735212852790127No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12120.20481726143098317No Hit
ATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAA11930.2016064297748869No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10230.172877936009815No Hit
ATTTATCGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10230.172877936009815No Hit
CTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT9670.16341443218132073No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT9410.1590206625466627No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9330.15766873342830634No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACATACT9170.15496487519159372No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG9080.15344395493344284No Hit
CTTCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA9060.15310597265385376No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTGTT8750.14786724732022302No Hit
CTACTGTACCTGTCTTTAATTCTGACTGGCAAACTCCCTCTTTTCCTCAC8720.1473602739008394No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8590.14516338908351034No Hit
CTCTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT8280.1399246637498796No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8180.1382347523519342No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT8070.13637584981419426No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8050.13603786753460517No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7990.1350239206958379No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7840.13248905359891983No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7750.13096813334076896No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7670.1296162042224126No Hit
GTGTATAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATC7630.12894023966323445No Hit
CTTCAAAGACTGTGTATTTACAGACATAGTTAATCATTACTTGTGGACAT7460.12606739028672725No Hit
GTACAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATCCC7460.12606739028672725No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7390.12488445230816549No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCC7380.12471546116837094No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA7270.122856558630631No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7050.1191387535551511No Hit
TATCGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCT6880.11626590417864392No Hit
GATAAAGGCATTAAACCTTATTATCCGGAACACATAGTTAATCATTACTT6750.11406901936131489No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6560.1108581877052186No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCCGGATAATAAGGTTTAATGCC6510.11001323200624591No Hit
TTCTTCTACTGTACCTGTCTTTAATTCTGACTGGCAAACTCCCTCTTTTC6470.10933726744706775No Hit
GTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6380.10781634718891689No Hit
CTATGGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG6330.1069713914899442No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT6310.1066334092103551No Hit
ATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTGGA6250.10561946237158785No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6120.10342257755425882No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTGTTGTGGTTTCACATTTCC6110.1032535864144643No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC6010.10156367501651888No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6000.10139468387672436No Hit
GATTAACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5990.10122569273692981No Hit
GCCTAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT5960.10071871931754618No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5960.10071871931754618No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5960.10071871931754618No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5930.10021174589816256No Hit
ATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTGGAAG5920.10004275475836802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)