FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591747
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG36960.624591252680622No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25160.4251817077230641No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT24380.41200039881908995No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCCGG20960.35420542900935703No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19240.3251389529646961No Hit
GTGTACAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATC16920.28593300853236264No Hit
TATCCATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA16530.27934235408037555No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT15400.26024635528359247No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA15170.25635955906831803No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG14190.23979842736845308No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG14080.23793952483071312No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12160.20549322599016134No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCACAG11840.20008550951673604No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACATACT11380.19231191708618717No Hit
ATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAA11220.18960805884947451No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10630.17963758160159662No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10220.17270894487002045No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10150.1715260068914587No Hit
ATTTATCGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10050.16983609549351328No Hit
CTTCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA10010.1691601309343351No Hit
CTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT9680.16358342332111528No Hit
CTCTTATATAAAATACCAGCCTTCCATAGAGTATGTAAATAATGCCTAGT9280.15682377772933365No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9090.15361294607323736No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT8700.1470222916212503No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT8570.14482540680392125No Hit
CTACTGTACCTGTCTTTAATTCTGACTGGCAAACTCCCTCTTTTCCTCAC8570.14482540680392125No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8560.14465641566412674No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTGTT8480.1433044865457704No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8330.1407696194488523No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8260.13958668147029052No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC8170.13806576121213965No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8170.13806576121213965No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCCGGATAATAAGGTTTAATGCC8030.13569988525501608No Hit
GTGTATAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATC7990.1350239206958379No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7710.13029216878159078No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7450.1258983991469327No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA7210.12184261179186376No Hit
CTTCAAAGACTGTGTATTTACAGACATAGTTAATCATTACTTGTGGACAT7150.1208286649530965No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7100.11998370925412381No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAAT7080.11964572697453474No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA6980.11795581557658932No Hit
GTACAATATGTTCCTGTGGTAATGTACCCCAACTTCCAATTACATATCCC6970.11778682443679478No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6910.11677287759802753No Hit
GCCTAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT6890.11643489531843844No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT6880.11626590417864392No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCC6730.11373103708172581No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC6440.10883029402768414No Hit
CTATGGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG6430.1086613028878896No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTGTTGTGGTTTCACATTTCC6270.10595744465117694No Hit
GATTAACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6270.10595744465117694No Hit
TTCTTCTACTGTACCTGTCTTTAATTCTGACTGGCAAACTCCCTCTTTTC6190.10460551553282062No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6180.10443652439302607No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT6120.10342257755425882No Hit
GTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6010.10156367501651888No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA6000.10139468387672436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)