FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606785
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42370.6982703923135872No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA30880.5089117232627701No Hit
TCCATAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA29800.4911129971901085No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA19820.3266395840371796No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT19280.31774022100084875No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG15790.260223967303081No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA15290.2519838163435154No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT12220.20138928945178275No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA12100.19941165322148702No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTGCACTTCAT12050.19858763812553049No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10520.17337277618925978No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10510.17320797317006847No Hit
GAATAACCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA10170.1676046705175639No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9850.1623309739034419No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9850.1623309739034419No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC9830.1620013678650593No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA8820.1453562629267368No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT8690.14321382367724977No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA8270.13629209687121469No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8190.13497367271768418No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8180.13480886969849287No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA8080.13316083950657975No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8010.13200721837224058No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC7980.13151280931466663No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7880.12986477912275354No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7780.12821674893084042No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7560.12459108250863155No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA7490.12343746137429237No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7350.12113021910561401No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7240.11931738589450959No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA7210.11882297683693564No Hit
CCCAATTACATATCCCATGAAGTGCAGGGAATAACCCCATCTTTTTGTTT7140.11766935570259648No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7130.11750455268340516No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT7080.11668053758744859No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT7040.11602132551068337No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7010.11552691645310943No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6990.1151973104147268No Hit
AATACGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCT6970.11486770437634418No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6900.113714083242005No Hit
GTCGATAAGATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA6830.1125604621076658No Hit
GTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTTCCTGTGGCAAT6820.1123956590884745No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT6800.11206605305009187No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6710.11058282587737008No Hit
TTCGAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGA6660.10975881078141353No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6510.10728676549354385No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC6510.10728676549354385No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA6410.10563873530163072No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6390.1053091292632481No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6360.10481472020567417No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT6290.10366109907133497No Hit
AATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCCTTAG6270.10333149303295237No Hit
CGATAAGATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGT6200.10217787189861317No Hit
CTTTTATATAAGATCCCCGCCTTCCATAGAGTGTGTAAATAATGTCGCGT6150.10135385680265663No Hit
CAATTACATATCCCATGAAGTGCAGGGAATAACCCCATCTTTTTGTTTTG6090.10036503868750876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)