FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606785
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42250.6962927560832914No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA28340.46705175638817703No Hit
TCCATAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA26410.4352447736842539No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT18100.298293464736274No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA16800.27686907224140345No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG16680.2748914360111077No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA16610.27373781487676857No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTGCACTTCAT11340.18688662376294735No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10990.18111851809125143No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10910.17980009393772095No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA10850.17881127582257306No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10630.1751856094003642No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10560.17403198826602503No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10440.1720543520357293No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC10210.16826388259432914No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9470.15606845917417206No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9130.15046515652166748No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA8910.14683949009945862No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8640.14238980858129321No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8540.1407417783893801No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA8470.1395881572550409No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8430.13892894517827567No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA8120.133820051583345No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT8040.13250162742981453No Hit
GAATAACCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA7850.1293703700651796No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7770.12805194591164912No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7760.12788714289245778No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7680.12656871873892728No Hit
GTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTTCCTGTGGCAAT7480.12327265835510107No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA7310.12047100702884876No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7040.11602132551068337No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7040.11602132551068337No Hit
GTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6990.1151973104147268No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6970.11486770437634418No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA6970.11486770437634418No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA6880.11338447720362238No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC6860.11305487116523975No Hit
AATACGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCT6670.10992361380060482No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6640.1094292047430309No Hit
CTCTATGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6610.10893479568545696No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT6600.10876999266626565No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT6570.1082755836086917No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC6570.1082755836086917No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6400.10547393228243943No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6310.1039907051097176No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT6240.10283708397537843No Hit
CTTTTATATAAGATCCCCGCCTTCCATAGAGTGTGTAAATAATGTCGCGT6240.10283708397537843No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT6210.1023426749178045No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG6170.10168346284103924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)