FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612599
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92691.513061562294421No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA23810.3886718718117398No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT23800.3885086328903573No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT22510.3674508120320144No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA20350.3321912050133938No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA18440.30101257102933565No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT16850.2750575825295177No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT16390.2675485921459225No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA15760.2572645400988248No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA15560.2539997616711748No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14500.23669643600462945No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT13710.22380056121541173No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT13710.22380056121541173No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA13160.21482242053937403No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT12760.20829286368407393No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT12620.2060075187847189No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT11770.19213221046720613No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11310.18462322008361098No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT11180.18250111410563843No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11170.18233787518425595No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11010.17972605244213588No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT10920.17825690214969334No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA10510.17156410637301073No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10470.17091115068748072No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC10410.1699317171591857No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA10360.16911552255227316No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC10040.16389187706803307No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA9770.15948442619070552No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA9580.15638288668443795No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC9500.1550769753133779No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9420.1537710639423179No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9260.15115924120019786No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9160.1495268519863728No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC9030.14740474600840028No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA8460.1381001274895976No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8420.1374471718040676No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC8410.1372839328826851No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAATCTAGCAGGCATAA8350.13630449935439007No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8320.13581478259024254No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA8270.13499858798333003No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCGAAACAG8060.13157057063429747No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA8050.13140733171291497No Hit
GTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAA8030.13108085387014998No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7990.13042789818461994No Hit
GGGTAAAGGGTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7960.12993818142047245No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC7930.12944846465632492No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT7690.12553073054314487No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7690.12553073054314487No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC7270.11867469584507973No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT7240.11818497908093223No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT7230.11802174015954972No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG7220.11785850123816721No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7190.11736878447401972No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7180.11720554555263721No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7120.11622611202434219No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAATCTAGCAGGC7100.11589963418157719No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC7070.11540991741742967No Hit
CTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT7020.11459372281051716No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6720.10969655516904206No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT6650.10855388271936454No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCGAAAC6600.10773768811245202No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA6570.10724797134830451No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC6540.10675825458415701No Hit
GCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT6530.1065950156627745No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6520.10643177674139201No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6520.10643177674139201No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6490.1059420599772445No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT6480.10577882105586199No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAATCCCAAAAGACCCACAATTCG6470.1056155821344795No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6460.10545234321309699No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAG6370.10398319292065446No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT6250.10202432586406442No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG6170.10071841449300441No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT6150.1003919366502394No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT6130.10006545880747438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)