FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561577
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32400.576946705438435No Hit
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTGGTTT30410.541510781246383No Hit
CTCTAAATGCAAATGAAAAAAGGAGATTAAAGCTAATTATGCCCGCTAGA18410.3278268162691848No Hit
CCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCGGG16990.3025408804135497No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTGGT16790.29897948099726307No Hit
GGATAATAAGGTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTT16590.29541808158097643No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15880.2827751136531589No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAA15840.28206283376990154No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14910.2655023264841687No Hit
GTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAAT14140.25179093873146513No Hit
TCCAAAGGGTATGCAAATAATGCCTGGTTTTGAAGTAATGATTAACTGAA14070.25054444893576483No Hit
GGATAAAGGTATTAAACCTTATTATCCTGAACATTCAGTTAATCATTACT12650.2252585130801297No Hit
TTATTATCCTGAACATTCAGTTAATCATTACTTCAAAACCAGGCATTATT12150.21635501453941308No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12090.21528659471452713No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11930.21243747518149783No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11510.2049585364072959No Hit
CTATTAATAATGTCTTCCTGCAAATGTATATCAGGAAAAGAGGGAGTTTG11300.2012190670201949No Hit
GTATACATGCATACAAAGGCAGTAAGGCAGGATAACCACATTGTGTAAAA11150.19854801745797993No Hit
GTATTAAACCTTATTATCCTGAACATTCAGTTAATCATTACTTCAAAACC11050.19676731774983663No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA10440.18590504953016238No Hit
TTCTTCTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCTCTTTTC10370.18465855973446205No Hit
GATATACATTTGCAGGAAGACATTATTAATAGATGTCAACAATTTGTGGG10220.18198751017224707No Hit
ATTTACAGGTAGTTTTCGGAAACACTGCTTGATTTTCATTACAATATGTT10050.17896032066840342No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTATC9900.17628927110618844No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9340.16631735274058587No Hit
CTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCTCTTTTCCTGAT9220.1641805130908139No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9090.1618656034702276No Hit
TTCCTAACCTTACCAAATATTTGCCATTGGATAAAGGTATTAAACCTTAT8810.1568796442874263No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAGATCAGACAACTATT8800.15670157431661197No Hit
ATTGACAACAGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT8760.15598929443335466No Hit
GGTTAGGAAAAAATCTAGCGGGCATAATTAGCTTTAATCTCCTTTTTTCA8720.15527701455009732No Hit
GTTTTATTAGGGTTCAAATGGATACCCAAAGATAAAAGAAAATTGGTAAC8710.15509894457928297No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8580.15278403495869666No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8550.15224982504625367No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT8510.15153754516299633No Hit
CAATAGGCCTATTTACAGGTAGTTTTCGGAAACACTGCTTGATTTTCATT8490.15118140522136767No Hit
TAATAAAACCAAGCGTTGGGGCTATTCCCTAAACTTCATGGGATATGTAA8260.14708579589263804No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCCAATGGCAAATATTTGGTA8150.1451270262136804No Hit
CATTTAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCTTCCTGC8140.14494895624286608No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8090.14405860638879442No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGTAGTTTTCGGAAAC8020.1428121165930941No Hit
CTTTTACACAATGTGGTTATCCTGCCTTACTGCCTTTGTATGCATGTATA7890.14049720697250778No Hit
CATTATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAA7720.13747001746866414No Hit
CTCAAGAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7650.1362235276729638No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7540.13426475799400617No Hit
GGGTAAAGGGTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7320.13034721863609086No Hit
ATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAAAG7200.1282103789863189No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7120.12678581921980425No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7110.1266077492489899No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7070.12589546936573257No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTTAGGGAA6990.12447090959921792No Hit
TCCCTAAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACA6990.12447090959921792No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT6970.12411476965758926No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAAA6900.12286827986188893No Hit
GTTTTGAAGTAATGATTAACTGAATGTTCAGGATAATAAGGTTTAATACC6770.12055337024130261No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6720.11966302038723096No Hit
GCTTTATTCTTCTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCT6670.1187726705331593No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC6610.11770425070827331No Hit
ATGTAAGACCTTGGGCAAGACCTGATTGGCGTTCATGGTGGTCTCCATGC6530.11627969094175866No Hit
GGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCGGGCATAATTAGCTT6520.11610162097094431No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6370.11343057140872934No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6320.11254022155465769No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCT6260.11147180172977168No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6150.10951303205081406No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAGAGACCGCCGTG6130.10915689210918539No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTAATGAAA6110.1088007521675567No Hit
TAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAAAGGA6100.10862268219674238No Hit
GGTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAA6080.10826654225511373No Hit
ATCCTGAACATTCAGTTAATCATTACTTCAAAACCAGGCATTATTTGCAT6020.10719812243022774No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC5980.10648584254697041No Hit
ATCTTTGGGTATCCATTTGAACCCTAATAAAACCAAGCGTTGGGGCTATT5970.10630777257615608No Hit
ATTAATAATGTCTTCCTGCAAATGTATATCAGGAAAAGAGGGAGTTTGCC5880.10470514283882708No Hit
TAATTAGCTTTAATCTCCTTTTTTCATTTGCATTTAGAGGGCCCACAAAT5830.10381479298475543No Hit
GATTAAAGCTAATTATGCCCGCTAGATTTTTTCCTAACCTTACCAAATAT5770.10274637315986944No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5760.1025683031890551No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5720.10185602330579778No Hit
CATTTGCAGGAAGACATTATTAATAGATGTCAACAATTTGTGGGCCCTCT5680.10114374342254046No Hit
GGCATAATTAGCTTTAATCTCCTTTTTTCATTTGCATTTAGAGGGCCCAC5650.10060953351009745No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTG5630.10025339356846881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)