FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561577
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32220.5737414459637771No Hit
CTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTGGTTT29750.5297581631726371No Hit
CTCTAAATGCAAATGAAAAAAGGAGATTAAAGCTAATTATGCCCGCTAGA19410.345633813350618No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAATAATGCCTGGT18610.33138821568547144No Hit
GGATAATAAGGTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTT18040.32123822734905455No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAA17060.30378737020925006No Hit
CCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCGGG16280.28989791248573216No Hit
GTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAAT15590.27761108449954325No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14630.2605163673013674No Hit
TCCAAAGGGTATGCAAATAATGCCTGGTTTTGAAGTAATGATTAACTGAA13750.2448462098697062No Hit
GTGCTGGTACGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13470.2398602506869049No Hit
CTATTAATAATGTCTTCCTGCAAATGTATATCAGGAAAAGAGGGAGTTTG12310.21920413407244244No Hit
GGATAAAGGTATTAAACCTTATTATCCTGAACATTCAGTTAATCATTACT11870.21136905535661185No Hit
TTATTATCCTGAACATTCAGTTAATCATTACTTCAAAACCAGGCATTATT11380.20264362678670955No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11360.2022874868450809No Hit
ATTTACAGGTAGTTTTCGGAAACACTGCTTGATTTTCATTACAATATGTT11240.20015064719530892No Hit
GTATTAAACCTTATTATCCTGAACATTCAGTTAATCATTACTTCAAAACC11180.19908222737042292No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11050.19676731774983663No Hit
TTCTTCTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCTCTTTTC10850.19320591833355No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10290.1832339999679474No Hit
GTATACATGCATACAAAGGCAGTAAGGCAGGATAACCACATTGTGTAAAA10240.18234365011387577No Hit
GTTTTATTAGGGTTCAAATGGATACCCAAAGATAAAAGAAAATTGGTAAC10200.1816313702306184No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9990.17789190084351744No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTATC9800.17450857139804515No Hit
CTATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATA9730.17326208160234483No Hit
GGTTAGGAAAAAATCTAGCGGGCATAATTAGCTTTAATCTCCTTTTTTCA9400.16738577256547188No Hit
CAATAGGCCTATTTACAGGTAGTTTTCGGAAACACTGCTTGATTTTCATT9250.1647147230032569No Hit
GATATACATTTGCAGGAAGACATTATTAATAGATGTCAACAATTTGTGGG9170.16329016323674225No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCCAATGGCAAATATTTGGTA9080.16168753349941326No Hit
CTTTTACACAATGTGGTTATCCTGCCTTACTGCCTTTGTATGCATGTATA8900.1584822740247553No Hit
GTTTTGAAGTAATGATTAACTGAATGTTCAGGATAATAAGGTTTAATACC8650.154030524754397No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8570.15260596498788234No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAGATCAGACAACTATT8150.1451270262136804No Hit
CATTTAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCTTCCTGC8120.1445928163012374No Hit
CTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCTCTTTTCCTGAT8080.14388053641798007No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7970.14192176673902243No Hit
TTCCTAACCTTACCAAATATTTGCCATTGGATAAAGGTATTAAACCTTAT7890.14049720697250778No Hit
TAATAAAACCAAGCGTTGGGGCTATTCCCTAAACTTCATGGGATATGTAA7810.1390726472059931No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGTAGTTTTCGGAAAC7800.13889457723517878No Hit
CATTATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAA7780.13853843729355012No Hit
CTCAAGAACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA7420.13212791834423418No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCT7260.12927879881120485No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7200.1282103789863189No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTAATGAAA7190.12803230901550455No Hit
GGGTAAAGGGTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7180.1278542390446902No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7060.12571739939491824No Hit
ATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAAAG6990.12447090959921792No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6940.12358055974514626No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6910.12304634983270327No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6880.12251213992026026No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTAAATGCAAATGAAAAAA6860.12215599997863161No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6780.12073144021211694No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6750.12019723029967397No Hit
TCCCTAAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACA6670.1187726705331593No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6550.11663583088338732No Hit
ATCTTTGGGTATCCATTTGAACCCTAATAAAACCAAGCGTTGGGGCTATT6480.11538934108768699No Hit
ATTGACAACAGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT6430.11449899123361533No Hit
GTATAGGCCCCTTACTCTGGGATCTAGCAGAGCTTGATGAAATGTTGTGG6390.113786711350358No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6380.11360864137954368No Hit
GCTTTATTCTTCTACTGTACCTGTCTTCAATCCTGAATGGCAAACTCCCT6280.11182794167140037No Hit
GGTTTAATACCTTTATCCAATGGCAAATATTTGGTAAGGTTAGGAAAAAA6270.11164987170058603No Hit
ATATAAGAGAGAAACTACACGCAGCGCATCATTTTGTGGGTCACCATATT6210.11058145187570004No Hit
GGCAAATATTTGGTAAGGTTAGGAAAAAATCTAGCGGGCATAATTAGCTT6200.1104033819048857No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6180.11004724196325703No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTTAGGGAA6170.10986917199244271No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCAC6130.10915689210918539No Hit
ATTAATAATGTCTTCCTGCAAATGTATATCAGGAAAAGAGGGAGTTTGCC6000.10684198248859907No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5990.10666391251778473No Hit
ATCCTGAACATTCAGTTAATCATTACTTCAAAACCAGGCATTATTTGCAT5970.10630777257615608No Hit
GTTTAGGGAATAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGGATAC5830.10381479298475543No Hit
CCTGTAAATAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5790.10310251310149811No Hit
CCTTACCACAGGAACATATTGTAATGAAAATCAAGCAGTGTTTCCGAAAA5740.10221216324742644No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5710.10167795333498346No Hit
GGGTATGCAAATAATGCCTGGTTTTGAAGTAATGATTAACTGAATGTTCA5690.10132181339335478No Hit
CCCCGAAGGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA5690.10132181339335478No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCAAAGGGTATGCAAA5680.10114374342254046No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAGAGACCGCCGTG5640.10043146353928313No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTAT5640.10043146353928313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)