FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725611
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53060.7312458052592918No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT49380.6805299258142449No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA47560.655447615871314No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT32870.45299754276051496No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG26510.36534727285005325No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA21580.2974045321804658No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC20310.27990204117633277No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT18790.2589541779272916No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18390.2534415823354387No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA18380.25330376744564237No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT17840.2458617633966409No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT17580.2422785762619365No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC17010.23442312754354605No Hit
ATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT16950.23359623820476813No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15390.2120971153965417No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATAACCC14620.20148536888222476No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGC14280.1967996626291498No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA13980.1926652159352601No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG13420.18494758210666598No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13320.18356943320870273No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATAACCCATGAA13280.18301817364951745No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG12830.1768165036086829No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT12810.17654087382909023No Hit
CAATTACATAACCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG12290.16937449955968142No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT12180.16785853577192186No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA12120.16703164643314392No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11930.16441316352701377No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC11810.1627593848494579No Hit
GTTTTATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC11580.15958964238414247No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11330.15614427013923438No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11210.1544904914616785No Hit
ATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGCATAA11140.15352578723310423No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA10910.1503560447677888No Hit
CATGTATACAAACCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10890.15008041498819616No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG10890.15008041498819616No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA10850.14952915542901085No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTATAATATGTT10820.1491157107596219No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10760.14828882142084396No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10360.14277622582899102No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10150.1398821131432682No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10070.13877959402489765No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGT10040.13836614935550867No Hit
GAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAATGT9960.13726363023713808No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACCAAAC9820.13533422177998955No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCA9820.13533422177998955No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA9700.13368044310243368No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACCAAACAG9480.13064851552691456No Hit
CCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCA9460.1303728857473219No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA9410.1296838112983403No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA9390.12940818151874764No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT9360.1289947368493587No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTAT9230.1272031432820065No Hit
AGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT9170.12637625394322854No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9060.12486029015546897No Hit
AATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAA9040.12458466037587633No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT9030.12444684548608001No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC9010.12417121570648736No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG8970.12361995614730208No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA8960.12348214125750573No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8940.12320651147791309No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8890.12251743702893148No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA8730.12031239879219031No Hit
CCTTTATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG8680.11962332434320869No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC8670.11948550945341237No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8630.11893424989422707No Hit
CCCAATTACATAACCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8610.11865862011463443No Hit
TGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGG8590.1183829903350418No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG8430.1161779520983006No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC8400.11576450742891164No Hit
CATTTGGATAAAACCTAGCAGGCATAACTAATTTTAGTCTCCTGTTTTCA8370.11535106275952266No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8340.1149376180901337No Hit
ACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTT8300.1143863585309484No Hit
GCTTACATCTATCAACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAA8260.1138350989717631No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT8250.11369728408196678No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC8230.11342165430237414No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8160.11245695007379988No Hit
AGTTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATATTTGCCCTTAG8130.1120435054044109No Hit
GGAACATATTATACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8130.1120435054044109No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC8120.11190569051461458No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8070.11121661606563295No Hit
GGATAAAACCTAGCAGGCATAACTAATTTTAGTCTCCTGTTTTCATTTAC7930.10928720760848443No Hit
GACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATAT7880.10859813315950281No Hit
ACATAACCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGG7750.10680653959215061No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA7710.10625528003296533No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA7690.10597965025337266No Hit
ATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAA7670.10570402047378002No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC7520.10363679712683518No Hit
TCTATCAACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7500.10336116734724252No Hit
CCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAA7480.10308553756764988No Hit
GATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACTT7430.10239646311866826No Hit
CTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT7420.10225864822887194No Hit
CTGCTAGGTTTTATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGG7380.10170738866968666No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7360.101431758890094No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7280.10032923977172342No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7280.10032923977172342No Hit
GTTGTGAGTATGCCCTGAGCCTGCGGGCTCCACCCCAACAGTCCCCCATG7280.10032923977172342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)