FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725611
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52860.7284895074633654No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT48680.6708828835285021No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA46110.6354644568508471No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT34490.4753235549075193No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG27190.3747186853562033No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT20790.2865171558865563No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA20070.276594483821221No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT19780.2725978520171276No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC19650.2708062584497754No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA19140.2637776990701629No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18560.2557844354619762No Hit
ATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT15570.2145777834128755No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT15550.21430215363328287No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC15380.21195930050674533No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATAACCC15240.21002989204959682No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15050.20741140914346667No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG14810.2041038517883549No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT14140.19487025417200124No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG13910.1917005117066858No Hit
GTTTTATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC13630.18784169479238874No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGC13120.18081313541277627No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12760.17585179938010861No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATAACCCATGAA12560.17309550158418216No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT12480.17199298246581157No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA12390.17075264845764468No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA12380.17061483356784832No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12140.16730727621273658No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTATAATATGTT11950.16468879330660643No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11560.15931401260454983No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11470.1580736785963829No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11180.15407704679228953No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC11150.15366360212290056No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA11060.15242326811473367No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11040.152147638335141No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC11030.15200982344534467No Hit
ATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGCATAA10700.147461932082066No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA10440.1438787449473616No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10410.14346530027797263No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG10270.1415358918208241No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10230.14098463226163882No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACCAAAC10220.1408468173718425No Hit
CCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCA10080.13891740891469395No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG9940.13698800045754544No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAACCAAACAG9870.13602329622897116No Hit
CAATTACATAACCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG9800.1350585920003969No Hit
CATGTATACAAACCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9800.1350585920003969No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGT9710.13381825799223No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA9600.13230229420447046No Hit
GAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAATGT9560.13175103464528515No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9480.13064851552691456No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCA9410.1296838112983403No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG9170.12637625394322854No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA9080.12513591993506162No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC9040.12458466037587633No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTAT9020.12430903059628368No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA8990.12389558592689472No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA8910.12279306680852413No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC8900.12265525191872781No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC8700.11989895412280133No Hit
GCTTACATCTATCAACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAA8640.11907206478402338No Hit
GGATAAAACCTAGCAGGCATAACTAATTTTAGTCTCCTGTTTTCATTTAC8550.11783173077585649No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT8550.11783173077585649No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG8480.11686702654728222No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC8390.1156266925391153No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA8380.11548887764931898No Hit
AGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACT8360.11521324786972634No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC8350.11507543297993002No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8320.11466198831054104No Hit
CATTTGGATAAAACCTAGCAGGCATAACTAATTTTAGTCTCCTGTTTTCA8290.11424854364115208No Hit
AATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAA8260.1138350989717631No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA8260.1138350989717631No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG8210.1131460245227815No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT8150.11231913518400356No Hit
ATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAA8090.11149224584522562No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8040.11080317139624399No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA8010.11038972672685501No Hit
GTTGTGAGTATGCCCTGAGCCTGCGGGCTCCACCCCAACAGTCCCCCATG8000.1102519118370587No Hit
TGATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGG7960.10970065227787341No Hit
GACTAAAATTAGTTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATAT7910.10901157782889179No Hit
ACAATATCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCATTT7840.10804687360031753No Hit
AGTTATGCCTGCTAGGTTTTATCCAAATGTTACTAAATATTTGCCCTTAG7820.10777124382072487No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT7790.1073577991513359No Hit
GGAACATATTATACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7730.10653090981255797No Hit
CTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT7580.10446368646561313No Hit
GCCCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTA7490.1032233524574462No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC7490.1032233524574462No Hit
CTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT7470.10294772267785356No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7450.10267209289826092No Hit
CCTTTATCTAAGGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG7410.10212083333907562No Hit
GATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTACTT7300.10060486955131606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)