FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535620
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44940.839027668869721No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT17500.3267241701206079No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14310.26716702139576565No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA13670.25521825174564056No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12530.23393450580635528No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12100.22590642619767748No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11510.21489115417646837No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11280.2105970650834547No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT11260.21022366603188827No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10960.20462268025839217No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10940.20424928120682573No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC10910.20368918262947613No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10880.20312908405212654No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10140.18931331914416938No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9080.1695231694111497No Hit
CTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC8920.16653597699861844No Hit
GGGTAAAGGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8880.16578917889548558No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG8730.16298868600873753No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8680.16205518837982152No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8550.15962809454463986No Hit
TTCTTCTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTC8200.1530936111422277No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTATAATATGTT8020.14973301967813002No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA7900.14749262536873156No Hit
TCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTGG7840.14637242821403232No Hit
GGAACATATTATACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7370.13759755050222172No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7160.13367686046077443No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7110.1327433628318584No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7060.13180986520294238No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTATACAAAAA7040.13143646615137597No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA7000.13068966804824317No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6980.13031626899667673No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6970.13012956947089355No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6940.12956947089354393No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6930.12938277136776072No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6910.12900937231619433No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6860.1280758746872783No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6850.1278891751614951No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6740.12583548037787984No Hit
GTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6590.12303498749113179No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6580.12284828796534857No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6550.12228818938799894No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6510.12154139128486613No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6510.12154139128486613No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6490.12116799223329973No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6420.11986109555281729No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6340.11836749934655166No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6330.11818079982076844No Hit
CAATTACATATCCCATGAAGTTAAGGGAATACCCCCATCTTTTTGTTTTG6240.11650050408871962No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6190.11556700645980358No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6170.11519360740823718No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6050.11295321309883874No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5960.11127291736678989No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5950.1110862178410067No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5900.11015272021209066No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5880.10977932116052426No Hit
GCTTTATTCTTCTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5860.10940592210895783No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5740.10716552779955939No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5620.10492513349016094No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5610.10473843396437772No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTTAGATACTGTTTACTT5560.10380493633546171No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5490.10249803965497928No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5400.10081774392293043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)