FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535620
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44920.8386542698181546No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16180.3020798327172249No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA13810.2578320451066054No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13750.2567118479519062No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12640.2359882005899705No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12110.2260931257234607No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11610.21675814943430044No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11260.21022366603188827No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11220.20947686792875544No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC10980.20499607930995856No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10720.20014189163959523No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT10130.18912661961838617No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9980.1863261267316381No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9950.18576602815428847No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9860.18408573242223966No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG9220.17213696277211457No Hit
CTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC8910.1663492774728352No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8860.1654157798439192No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8590.16037489264777266No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTATAATATGTT8330.15552070497740936No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8190.15290691161644449No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8070.15066651730704606No Hit
TTCTTCTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTC8000.1493596206265636No Hit
GGGTAAAGGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7960.1486128225234308No Hit
TCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTGG7600.14189163959523543No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA7510.1402113438631866No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7410.13834434860535452No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7270.13573055524438968No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTATACAAAAA7130.13311676188342483No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7000.13068966804824317No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6900.1288226727904111No Hit
GGAACATATTATACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6870.1282625742130615No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6780.12658227848101267No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6600.12322168701691498No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6570.12266158843956536No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6570.12266158843956536No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6550.12228818938799894No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6520.12172809081064935No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6420.11986109555281729No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6320.11799410029498525No Hit
GTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6290.11743400171763563No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6250.11668720361450281No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6070.11332661215040514No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6030.11257981404727233No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6030.11257981404727233No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5970.11145961689257308No Hit
GCTTTATTCTTCTACTGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5880.10977932116052426No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5870.10959262163474105No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA5850.10921922258317464No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5840.10903252305739145No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT5820.10865912400582502No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5820.10865912400582502No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG5620.10492513349016094No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5550.10361823680967851No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5510.10287143870654569No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5480.10231134012919609No Hit
CAATTACATATCCCATGAAGTTAAGGGAATACCCCCATCTTTTTGTTTTG5440.10156454202606327No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTTAGATACTGTTTACTT5380.10044434487136403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)