FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375792
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19740.5252905862817729No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTCACAGTTAATGAAAAAA19160.5098565163707584No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC18220.4848426789287691No Hit
CTGTCGATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTG16230.4318878528547707No Hit
AGCGTGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTAGAA13240.35232256141695406No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10440.2778132583982629No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10430.2775471537446247No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG10060.2677012815600119No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9640.25652488610720825No Hit
CTCTCGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA8840.2352365138161536No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT8420.22406011836334996No Hit
AAGCCATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGT8380.22299569974879718No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8250.21953633925150084No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA8110.2158108741005663No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCATAATGATTAACCT8110.2158108741005663No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7990.2126176182569081No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7860.2091582577596117No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7650.20357006003320988No Hit
CTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTAT7640.20330395537957166No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7320.19478860646314983No Hit
CCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAGTCTTTGA7020.18680546685400434No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6920.18414442031762251No Hit
CCGAACATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT6890.18334610635670798No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6790.18068505982032615No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6780.18041895516668796No Hit
ACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCC6600.17562907140120065No Hit
GGACAAATTGGAGGACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTG6570.17483075744028612No Hit
CTCCAAAAGACACCAGATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6530.1737663388257334No Hit
GGGTTGAGTTGACGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC6460.1719036062502661No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6430.17110529228935156No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATTA6300.16764593179205517No Hit
CTCCCACCTGGTTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC6210.16525098990931153No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6100.1623238387192915No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG6080.16179162941201514No Hit
AGGTAGGAGTGGGATCATTCGGGCCAGGGTTCACTCCACCACACGGCAGT5840.15540511772469878No Hit
GTCCGAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5730.15247796653467877No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5620.14955081534465875No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5600.1490186060373824No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5520.14688976880827692No Hit
AATCTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCC5440.14476093157917144No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5310.14130157108187508No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5270.14023715246732235No Hit
ATTTTGTGGTCTGTAAGCGTGAGGAGTGCGAATCCACACTCCAAAAGACA5230.13917273385276963No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5080.13518116404819688No Hit
ATTGACAACAGTGCCAGCAGCGCCACCTCCTGCCTCCACCAATCGGCAGT4980.13252011751181506No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA4800.12773023374632775No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4780.1271980244390514No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT4770.1269319197854132No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4720.1256013965172223No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4580.12187593136628774No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGGT4570.12160982671264955No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4560.12134372205901137No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4560.12134372205901137No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT4560.12134372205901137No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4400.11708604760080044No Hit
AAGCCATGTCAATGTTAACATGGGCCTAAAAACCAGACAATTATTGTGGT4370.1162877336398859No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC4360.11602162898624771No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4350.11575552433260954No Hit
GTTGACGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGCAT4340.11548941967897135No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTCACAGTTAATG4320.11495721037169497No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4300.11442500106441861No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4300.11442500106441861No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4290.11415889641078042No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCGA4260.11336058244986588No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4250.11309447779622771No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4180.11123174522076043No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4180.11123174522076043No Hit
CAATAGGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT4120.10963511729893131No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4120.10963511729893131No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4080.1085706986843786No Hit
ATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT4050.10777238472346404No Hit
ACTCACCCCAACACAAAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAA4040.10750628006982586No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4040.10750628006982586No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4010.10670796610891133No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3960.1053774428407204No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3940.10484523353344403No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3910.1040469195725295No Hit
GCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGT3880.10324860561161493No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA3860.10271639630433857No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3850.10245029165070038No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT3840.1021841869970622No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3830.10191808234342402No Hit
TTATCAACAGATGTCAACAATTTGTAGGCCCTCTCACAGTTAATGAAAAA3830.10191808234342402No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT3810.10138587303614767No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3760.10005534976795674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)