FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375792
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19640.5226295397453911No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTCACAGTTAATGAAAAAA16580.4412015157321071No Hit
CTGTCGATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTG15550.4137927364073743No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC14000.37254651509345593No Hit
AGCGTGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATACTCTAGAA13120.34912930557329586No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA11560.3076169796057393No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11270.29989994465023206No Hit
CTCTCGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA10240.2724911653254992No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9580.2549282581853792No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT8850.2355026184697918No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8620.2293822114361136No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8350.22219738578788265No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8300.22086686251969173No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8090.21527866479328991No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7750.20623110656959168No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA7680.2043683739941244No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCATAATGATTAACCT7670.20410226934048623No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7570.2014412228041044No Hit
CTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTAT7560.2011751181504662No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG7490.19931238557499895No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6900.18361221101034614No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6880.1830800017030698No Hit
CTCCAAAAGACACCAGATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6810.1812172691276025No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6580.1750968620939243No Hit
AAGCCATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGT6470.1721697109039043No Hit
CTCCCACCTGGTTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC6370.16950866436752246No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6180.164452675948397No Hit
ATTTTGTGGTCTGTAAGCGTGAGGAGTGCGAATCCACACTCCAAAAGACA5950.15833226891471877No Hit
CCGAACATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT5890.15673564099288967No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5820.1548729084174224No Hit
GGGTTGAGTTGACGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC5800.15434069911014606No Hit
GGACAAATTGGAGGACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTG5740.15274407118831695No Hit
ACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCC5720.15221186188104058No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5550.14768808276919146No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5530.1471558734619151No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5510.14662366415463873No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATTA5480.1458253501937242No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG5460.14529314088644782No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5230.13917273385276963No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5160.13731000127730233No Hit
AATCTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCC5130.1365116873163878No Hit
AGGTAGGAGTGGGATCATTCGGGCCAGGGTTCACTCCACCACACGGCAGT5070.1349150593945587No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4950.1317218035509005No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4930.1311895942436241No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4860.12932686166815685No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4730.12586750117086046No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4720.1256013965172223No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4700.12506918720994592No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4690.12480308255630776No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4660.1240047685953932No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4640.12347255928811683No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA4640.12347255928811683No Hit
GTCCGAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4610.12267424532720228No Hit
ATTGACAACAGTGCCAGCAGCGCCACCTCCTGCCTCCACCAATCGGCAGT4600.12240814067356409No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4520.12027930344445864No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4450.11841657086899136No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4380.11655383829352409No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4370.1162877336398859No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4320.11495721037169497No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4310.1146911057180568No Hit
CCTGATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAGTCTTTGA4280.11389279175714226No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT4270.11362668710350407No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4100.10910290799165497No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC4040.10750628006982586No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA4020.10697407076254949No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGGT4010.10670796610891133No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG4000.10644186145527314No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3990.10617575680163495No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC3980.10590965214799675No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3980.10590965214799675No Hit
ATTAATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT3940.10484523353344403No Hit
GTTGACGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGCAT3940.10484523353344403No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTCACAGTTAATG3910.1040469195725295No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG3850.10245029165070038No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3830.10191808234342402No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3830.10191808234342402No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG3780.1005875590752331No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3760.10005534976795674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)