FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463816
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA45440.9796988460941408No Hit
CATTTACACCAAGACATAATCCAAAAATGTGAACAATTTGTAGGCCCACT18080.38980975214309127No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14380.31003673870672854No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT14190.3059402866654018No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTCT13730.2960225606706108No Hit
GGATAAGGGTATCAAACCTTATTACCCAGAACATCTAGTTAATCATTACT12460.2686410128154268No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCTTACATTTCAT12010.258938889559653No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT11210.24169067043827724No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11180.24104386222122565No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTC10690.23047932800938303No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10480.2259516704900219No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10460.22552046501198753No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTG10000.21560273901719645No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9880.21301550614899012No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA9250.19943253359090674No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8850.19080842403021886No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.18778998568397814No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8610.18563395829380616No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8550.184340341859703No Hit
CACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGG8370.18045949255739344No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8290.1787346706452559No Hit
ACTAATGACTCTAGCTACCTGGGTGGGTGGTAATTTGGAAGATCCAATAT8150.17571623229901512No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTCGGTGGGCGTTC8100.17463821860392914No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8030.17312899943080878No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACT7820.16860134191144766No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7740.16687651999931005No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATCAAACCTTAT7690.1657985063042241No Hit
GTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTG7660.1651516980871725No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7500.16170205426289735No Hit
CTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT7440.1604084378287942No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7420.15997723235075978No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA7360.1586836159166566No Hit
GGGTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATT7220.15566517757041584No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGT7180.15480276661434705No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7060.15221553374614072No Hit
ATGTAAGACCTTGGGCAAGACTCGGTGGGCGTTCACGGTGGTCTCCATGC7050.1519999310071235No Hit
AATATACATTTACACCAAGACATAATCCAAAAATGTGAACAATTTGTAGG7040.1517843282681063No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6970.15027510909498595No Hit
CACCAAGACATAATCCAAAAATGTGAACAATTTGTAGGCCCACTAACAGT6810.1468254652707108No Hit
ATCCAAAAATGTGAACAATTTGTAGGCCCACTAACAGTCAATGAAAAAAG6770.14596305431464202No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6730.14510064335857323No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6690.14423823240250444No Hit
TACCCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6650.14337582144643565No Hit
ATCTAGAAGATCACGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6580.1418666022733153No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCGAAAAC6330.13647653379788538No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6270.13518291736378218No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATACCCACATTGTGTGAAA6270.13518291736378218No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6260.134967314624765No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA6210.13388930092967902No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTTTCA5990.1291460406713007No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5630.12138434206668161No Hit
GTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGTATGATGTGAT5630.12138434206668161No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5600.12073753384963003No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5540.11944391741552683No Hit
GCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTC5510.11879710919847526No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5430.11707228728633767No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC5410.11664108180830328No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5390.1162098763302689No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5360.11556306811321732No Hit
CCCACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTA5350.11534746537420011No Hit
GTATCTAGAAGATCACGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA5300.11426945167911412No Hit
TCCTAGTACAAAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAAC5250.11319143798402816No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5190.11189782154992496No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG5190.11189782154992496No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5180.11168221881090777No Hit
GACATAGCCCATGAAATGTAAGGAGTAACCCCATCTTTTTGTTTTGTTAG5150.11103541059385617No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5080.10952619142073582No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT5060.10909498594270141No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5030.10844817772564982No Hit
TTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT5010.10801697224761544No Hit
GTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5010.10801697224761544No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4980.10737016403056385No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4960.10693895855252945No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4890.10542973937940907No Hit
GGCATAATCAATTGCAGTCTTCTTTTTTCATTGACTGTTAGTGGGCCTAC4890.10542973937940907No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4850.1045673284233403No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAGGAGTAACCCCAT4790.10327371198923711No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4780.10305810925021991No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4780.10305810925021991No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4780.10305810925021991No Hit
GTTCTGGGTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTA4760.10262690377218552No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTCTTGTGGTTTCACATTTCTT4750.10241130103316833No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4730.10198009555513395No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4710.10154889007709955No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4680.10090208186004795No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4670.10068647912103075No Hit
CTCTTTTCCTAATATACATTTACACCAAGACATAATCCAAAAATGTGAAC4660.10047087638201356No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG4640.10003967090397917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)