FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463816
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA45920.9900477775669662No Hit
CATTTACACCAAGACATAATCCAAAAATGTGAACAATTTGTAGGCCCACT17950.38700691653586766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14380.31003673870672854No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTCT13490.29084809493419805No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT13430.28955447850009486No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13220.28502682098073373No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT13190.28438001276368213No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13190.28438001276368213No Hit
GGATAAGGGTATCAAACCTTATTACCCAGAACATCTAGTTAATCATTACT12690.2735998758128223No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCTTACATTTCAT12650.27273746485675354No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11920.2569984649084982No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11220.24190627317729443No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10390.22401124583886714No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9580.20654742397847423No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA9290.20029494454697552No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTG9060.19533608154958002No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9030.19468927333252842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8780.1892992048570985No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8420.18153750625247944No Hit
GGGTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATT8380.18067509529641065No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8310.17916587612329027No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8260.1780878624282043No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8240.17765665695016988No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTC8000.17248219121375719No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA7840.16903254738948204No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7790.16795453369439606No Hit
GTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTG7600.16385808165306934No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7520.16213325974093173No Hit
ACTAATGACTCTAGCTACCTGGGTGGGTGGTAATTTGGAAGATCCAATAT7270.15674319126550185No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGT7130.15372475291926108No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATCAAACCTTAT7110.1532935474412267No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTCGGTGGGCGTTC7090.1528623419631923No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7080.1526467392241751No Hit
CACTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGG7070.1524311364851579No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6840.1474722734877624No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6760.1457474515756248No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6700.14445383514152163No Hit
AATATACATTTACACCAAGACATAATCCAAAAATGTGAACAATTTGTAGG6620.14272901322938408No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6490.13992617762216053No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTTTCA6380.13755454749297136No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6180.13324249271262742No Hit
GTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGTATGATGTGAT6140.13238008175655863No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG6060.13065525984442108No Hit
ATCCAAAAATGTGAACAATTTGTAGGCCCACTAACAGTCAATGAAAAAAG6060.13065525984442108No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6050.13043965710540387No Hit
GTTTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6020.12979284888835227No Hit
GTTCTGGGTAATAAGGTTTGATACCCTTATCCAATGGCAAATATTTGGTA5940.1280680269762147No Hit
CTAACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5920.12763682149818031No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA5890.12699001328112872No Hit
TACCCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5840.12591199958604274No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5830.12569639684702555No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACT5820.12548079410800836No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5790.12483398589095676No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5790.12483398589095676No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT5730.1235403694568536No Hit
ATGTAAGACCTTGGGCAAGACTCGGTGGGCGTTCACGGTGGTCTCCATGC5730.1235403694568536No Hit
GGCATAATCAATTGCAGTCTTCTTTTTTCATTGACTGTTAGTGGGCCTAC5680.1224623557617676No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCGAAAAC5670.1222467530227504No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATACCCACATTGTGTGAAA5470.11793469824240647No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5440.11728789002535488No Hit
TTGATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT5420.1168566845473205No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5310.11448505441813132No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5270.11362264346206255No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5240.11297583524501095No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5200.11211342428894217No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5160.11125101333287338No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTG5080.10952619142073582No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5060.10909498594270141No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATCAAAC5060.10909498594270141No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAAGAATGT5040.10866378046466701No Hit
CACCAAGACATAATCCAAAAATGTGAACAATTTGTAGGCCCACTAACAGT5030.10844817772564982No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4930.10629215033547786No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4910.10586094485744346No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4860.1047829311623575No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT4830.1041361229453059No Hit
ATCTAGAAGATCACGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4790.10327371198923711No Hit
TTCCCTGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4780.10305810925021991No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC4750.10241130103316833No Hit
AGATCACATCATACAAAAAATCAAAGAATGTTTTCGAAAACTTCCTGTTA4720.10176449281611673No Hit
ATATTTGCCATTGGATAAGGGTATCAAACCTTATTACCCAGAACATCTAG4710.10154889007709955No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4710.10154889007709955No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4700.10133328733808235No Hit
ATGTGGGTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCGAAGC4690.10111768459906513No Hit
TCCTAGTACAAAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAAC4640.10003967090397917No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4640.10003967090397917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)