FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353035
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT19250.5452717152690243No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17090.4840879799453312No Hit
CATATAAACCCTCTCAAAACAAAAAGATGGGGGTACTCTTTACATTTCAT15260.43225175974053565No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13490.382115087739176No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13400.37956576543402215No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9500.2690951322106873No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9420.26682906793943945No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8930.25294942427804606No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8640.24473494129477247No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8600.24360190915914853No Hit
GGTTTATATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA8560.24246887702352457No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7540.21357655756511396No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7180.20337926834449843No Hit
GTTTATATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7050.1996969139037206No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA6990.19799736570028464No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6430.18213491580154942No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6380.1807186256320195No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6280.17788604529295962No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA6110.17307065871655786No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTTGCGA6040.17108785247921596No Hit
GACGTAGAAGGGGACGAGAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCC5880.16655572393672016No Hit
CATTTAAACCCTCTCAAAACAAAAAGATGGGGGTACTCTTTACATTTCAT5680.16089056325860043No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5550.1572082088178226No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5520.15635843471610464No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5490.15550866061438667No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5450.15437562847876274No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5430.15380911241095074No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5410.15324259634313878No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTAAG5190.14701091959720708No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5160.14616114549548911No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5050.14304530712252328No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5050.14304530712252328No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5030.14247879105471128No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCTTACTGAAGGAAAGAAGT5000.14162901695299332No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4880.1382299205461215No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4840.13709688841049755No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4680.13256475986800176No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC4630.13114846969847183No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4620.13086521166456583No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4530.12831588935941196No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4530.12831588935941196No Hit
CCCATTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTT4480.12689959918988203No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4430.1254833090203521No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4400.12463353491863412No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4380.12406701885082215No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4380.12406701885082215No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4350.1232172447491042No Hit
GGGTATACATATAAACCCTCTCAAAACAAAAAGATGGGGGTACTCTTTAC4240.12010140637613834No Hit
CATACAGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC4230.11981814834223237No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4200.1189683742405144No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4200.1189683742405144No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC4180.11840185817270242No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4150.11755208407098446No Hit
GCTCTATATCGCGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4150.11755208407098446No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4120.1167023099692665No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4100.11613579390145454No Hit
GTGTAAACAATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4060.11500276176583059No Hit
ATACAGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCA4030.11415298766411262No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG4020.11386972963020664No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4000.11330321356239467No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA3930.11132040732505276No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT3910.11075389125724078No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3900.11047063322333481No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3850.10905434305380486No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3810.10792131091818091No Hit
GTAATGGGCCATCAGCGCATGCGTGGAACCTTTCAGGCTCCTCTGCCGAT3750.106221762714745No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTTTCGCAAACTTACA3750.106221762714745No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3720.10537198861302703No Hit
ATATAAACCCTCTCAAAACAAAAAGATGGGGGTACTCTTTACATTTCATG3720.10537198861302703No Hit
CATGTATTCAATCTAAACAGGCTTTCACTTTTTCGCAAACTTACAAGGCC3680.10423895647740308No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGTAATGG3670.10395569844349711No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCATGTATTGTTTACAC3660.10367244040959112No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAACAGGCTTTCACTTT3650.10338918237568512No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3610.10225615024006117No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3610.10225615024006117No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA3590.10168963417224922No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3570.10112311810443725No Hit
CTCTATGGAAGGCGGGTATATTATATAAAAGAGAAACTACACATAGCGCC3540.10027334400271928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)