FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352888
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20360.5769535943415475No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14970.4242139148965111No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12020.3406179864432908No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT11110.31483076783568725No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11010.3119970075491374No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10100.28620978894153387No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9420.2669402189929949No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9090.2575888100473805No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9060.2567386819614155No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8820.2499376572736959No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7650.21678266192106274No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7380.2091315091473782No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7320.2074312529754483No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7180.2034639885742785No Hit
CATTTAAACCCTAACAAAACGAAAAGGTGGGGCTACTCTTTACATTTCAT6670.18901181111287435No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6500.1841944186257396No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6460.18306091451111967No Hit
CTGTAATGTCAATGACCGACCTTGAGGCATATTTCAAAGACTGTTTGTTT6270.17767676996667497No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6140.17399288159416018No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6120.17342612953685022No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6090.17257600145088528No Hit
ACATAGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5890.1669084808777856No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAATGACCGACCT5860.16605835279182063No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5850.16577497676316563No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5620.15925732810410104No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5520.15642356781755118No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5500.1558568157602412No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5440.1541565595883113No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGTTGGGCGTTC5350.15160617533041645No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5280.14962254312983156No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5270.1493391671011766No Hit
ATGTAAGACCTTGGGCAAGAATCGTTGGGCGTTCACGGTGGTCTCCATGC5260.1490557910725216No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5240.14848903901521163No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5190.1470721588719367No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5120.1450885266713518No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5110.14480515064269683No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4980.14112126227018204No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4800.13602049375439232No Hit
CTCTTGGACTCTCTGTAATGTCAATGACCGACCTTGAGGCATATTTCAAA4680.13261998141053252No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4670.13233660538187753No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4660.13205322935322256No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4640.13148647729591256No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4530.12836934098070776No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4510.1278025889233978No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4430.12553558069415793No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4350.12326857246491806No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4320.12241844437895308No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4300.12185169232164313No Hit
GCTTTGGGGCATGGACATAGATCCTTATAAAGAATTTGGAGCTACTGTGG4240.12015143614971323No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4220.11958468409240326No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4200.11901793203509328No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4160.11788442792047334No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4080.11561741969123349No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4020.11391716351930357No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3990.11306703543333863No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3990.11306703543333863No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3980.11278365940468364No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAGGGAAT3970.11250028337602865No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3910.11080002720409875No Hit
GTAAAGAGTAGCCCCACCTTTTCGTTTTGTTAGGGTTTAAATGTATACCC3900.11051665117544378No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC3880.1099498991181338No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3880.1099498991181338No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3840.10881639500351387No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTGTTCCTGTC3830.10853301897485888No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3820.10824964294620389No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCATTGACATTACAGAGAGTCCAA3820.10824964294620389No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3800.10768289088889393No Hit
ATCTAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3750.106266010745619No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3750.106266010745619No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3710.10513250663099907No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3710.10513250663099907No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA3700.10484913060234409No Hit
GCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACG3660.10371562648772414No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT3650.10343225045906916No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC3630.1028654984017592No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG3600.10201537031579425No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3590.10173199428713925No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3590.10173199428713925No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3590.10173199428713925No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3570.1011652422298293No Hit
GTATCCAGAAGATCTCGTACGGAAGGAAAGAAGTCAGAAGGCAAAAACGA3560.1008818662011743No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT3550.10059849017251933No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3540.10031511414386435No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3530.10003173811520935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)