FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419093
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34900.8327507259725168No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA20650.49273073041067256No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13410.3199767116129355No Hit
GTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATACGTTGACAGACT12020.28680984888795563No Hit
CATATAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9710.2316908180284567No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9360.22333944971641137No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT8460.20186450262829492No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8200.19566062902506126No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7790.1858775975738082No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7470.1782420608313668No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7430.1772876187385616No Hit
CACTCATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT7340.17514012402974996No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA7200.17179957670493184No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6930.1653570925784969No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6850.16344820839288654No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT6770.16153932420727618No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6490.15485822955763995No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6210.14817713490800372No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT6140.14650686124559464No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6140.14650686124559464No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6090.14531380862958818No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6050.144359366536783No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5960.14221187182797135No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5960.14221187182797135No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5660.13505355613193254No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5630.13433772456232865No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5620.13409911403912736No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5580.13314467194632218No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTGAA5540.132190229853517No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAAT5510.1314743982839131No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGT5370.12813385095909502No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5340.12741801938949113No Hit
CTCTTGGACTCTCTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAA5290.12622496677348463No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5170.12336164049506912No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5160.1231230299718678No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5140.12264580892546523No Hit
CACCAATTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGT5010.11954387212384841No Hit
GAATCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC4980.11882804055424452No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATACG4910.11715776689183545No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4870.11620332479903028No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4870.11620332479903028No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4800.11453305113662124No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT4750.11333999852061476No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC4740.11310138799741346No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4500.1073747354405824No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4440.10594307230137465No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4430.10570446177817333No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4390.10475001968536816No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4340.10355696706936171No Hit
CATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4340.10355696706936171No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4300.1026025249765565No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4220.10069364079094616No Hit
CTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTC4210.10045503026774487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)