FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419093
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34860.8317962838797117No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18950.4521669414664526No Hit
GTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATACGTTGACAGACT12170.29038900673597506No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT12080.2882415120271634No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8830.21069309198674277No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8410.20067145001228842No Hit
CACTAATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT7870.18778648175941856No Hit
CATATAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7690.18349149234179526No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7280.1737084608905422No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7240.17275401879773702No Hit
CACTCATATGGGCCTAAAGTTCCGGCAACTATTGTGGTTTCACATTTCTT7150.17060652408892538No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7050.16822041885691244No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6990.16678875571770466No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6990.16678875571770466No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA6950.16583431362489948No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6800.16225515577688007No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6570.1567671137432503No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6290.15008601909361408No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT6250.1491315770008089No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6140.14650686124559464No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAAT6110.14579102967599075No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6020.14364353496717913No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT5930.14149604025836748No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5900.1407802086887636No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5880.140302987642361No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5830.13910993502635455No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5670.13529216665513383No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5520.1317130088071144No Hit
CTCTTGGACTCTCTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAA5520.1317130088071144No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5400.12884968252869888No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5390.1286110720054976No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT5300.12646357729668595No Hit
ATATGAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5160.1231230299718678No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5070.12097553526305618No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC4950.11811220898464063No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTTTGT4830.1152488827062251No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4620.11023806171899793No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4540.10832917753338758No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4490.1071361249173811No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4420.10546585125497206No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATACG4420.10546585125497206No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4410.10522724073177075No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4310.10284113549975782No Hit
CACCAATTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCTGT4310.10284113549975782No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4300.1026025249765565No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4260.10164808288375134No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4260.10164808288375134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)