FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002488471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401207
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT22950.5720239178279541No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT21760.5423634183850232No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA21360.5323935026058867No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT21230.5291532799776674No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG21080.5254145615604913No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG20290.5057239778966968No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA19170.47780821371511467No Hit
GTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTTCCTGTGGCAAG18040.44964320163905414No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18040.44964320163905414No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA17480.43568531954826306No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT17210.428955626397346No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA16800.41873646272373116No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT16540.41225601746729246No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA14790.3686376359335704No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG13900.34645457332499185No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT13520.3369831533348122No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA12890.32128053598267226No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG12300.306574910208446No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC12070.30084220863544253No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11990.29884822547961526No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA11950.29785123390170165No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG11740.29261702811765494No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG11370.28339485602195374No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA11190.27890839392134237No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC11040.27516967550416616No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10700.2666952470919002No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA10680.26619675130294335No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC10360.2582208186796342No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT10330.257473074996199No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT10260.2557283397348501No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10250.25547909184037165No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9990.24899864658393298No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9900.2467554155336273No Hit
GAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTTCCTGTGGC9520.23728399554344767No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9460.23578850817657718No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA9380.2337945250207499No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG9090.22656633608087595No Hit
GCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGT8990.22407385713609185No Hit
GGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACA8940.2228276176636998No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT8790.2190888992465236No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG8280.20637725662812464No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA8240.205380265050211No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8230.2051310171557326No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG8050.2006445550551212No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8030.20014605926616433No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8020.19989681137168597No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC8010.19964756347720752No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7970.1986505718992939No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC7930.19765358032138025No Hit
GCCCCAACCAGTGGGGGTTGCATCAGCAAACACTTGGCAAAGACCTGACC7890.19665658874346661No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT7850.19565959716555292No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG7830.19516110137659612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7770.19366561400972565No Hit
CATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAA7700.19192087874837677No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7660.19092388717046313No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7620.18992689559254947No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC7590.18917915190911425No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCTCTAT7580.18892990401463583No Hit
CTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTG7520.18743441664776536No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7510.18718516875328695No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG7480.18643742506985173No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT7470.1861881771753733No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7450.18568968138641648No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC7440.18544043349193806No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7240.18045547560236985No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7210.17970773191893463No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAATATG7000.17447352613488798No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG6960.17347653455697432No Hit
TTCGAAAACTTCCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGA6890.17173179929562543No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6770.16874082456188452No Hit
GGGTAACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAAT6690.16674684140605722No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6610.16475285825022992No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6600.1645036103557515No Hit
GTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA6440.16051564404409693No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6360.15852166088826963No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA6210.15478294247109348No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6150.153287455104223No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6140.1530382072097446No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA6110.15229046352630937No Hit
GGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA6090.15179196773735254No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC6080.15154271984287412No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6080.15154271984287412No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCATCAGCAAACACTTGGC6060.15104422405391732No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6050.15079497615943888No Hit
CTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTC5970.1488009930036116No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5940.14805324932017636No Hit
GTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5670.14132355616925926No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5620.1400773166968672No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCTC5570.13883107722447516No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5520.13758483775208308No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATA5510.1373355898576047No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5500.13708634196312627No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5480.13658784617416944No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5460.1360893503852126No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5320.13259987986251484No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5300.13210138407355804No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT5290.13185213617907962No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGATTCAAGATG5280.1316028882846012No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAATTTCCGTCCGAAGGTTT5260.1311043924956444No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5140.12811341776190344No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5050.12587018671159778No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5040.12562093881711933No Hit
TTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG5040.12562093881711933No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5020.12512244302816253No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4990.12437469934472728No Hit
ACACGTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA4970.12387620355577046No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC4970.12387620355577046No Hit
TGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT4970.12387620355577046No Hit
ATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAAATAGA4970.12387620355577046No Hit
ATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGATT4910.1223807161889No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4880.12163297250546476No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT4830.12038673303307271No Hit
ATCTTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4820.12013748513859429No Hit
TATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTTCCT4790.11938974145515906No Hit
ATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCAA4790.11938974145515906No Hit
ACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACT4740.118143501982767No Hit
GGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4730.11789425408828859No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAATATGGA4680.11664801461589652No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC4660.1161495188269397No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG4650.1159002709324613No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG4600.11465403146006925No Hit
GCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGG4560.1136570398821556No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4530.11290929619872037No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCAC4490.11191230462080672No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4490.11191230462080672No Hit
CCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTG4480.1116630567263283No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC4470.1114138088318499No Hit
GGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCC4440.11066606514841465No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT4430.11041681725393626No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCATCAGCAAACA4420.11016756935945783No Hit
ACCACATTGTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTT4410.10991832146497943No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTC4390.1094198256760226No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA4360.10867208199258738No Hit
GGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATT4320.10767509041467373No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTA4320.10767509041467373No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACAT4310.10742584252019531No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4300.1071765946257169No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA4270.10642885094228167No Hit
CCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC4260.10617960304780324No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA4260.10617960304780324No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG4250.10593035515332484No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC4230.10543185936436802No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTAT4200.10468411568093279No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA4200.10468411568093279No Hit
GGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCA4180.10418561989197596No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4150.10343787620854072No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG4140.10318862831406232No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4130.1029393804195839No Hit
CATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGC4120.1026901325251055No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG4110.10244088463062709No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC4100.10219163673614869No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4080.10169314094719184No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4040.1006961493692782No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA4030.1004469014747998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)