FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588979
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT87951.4932620687664586No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT49940.8479079899283336No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAA46910.7964630317889092No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTG45010.7642038171140227No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGT42450.7207387699731229No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC25120.42650077507007894No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG24840.42174678553904305No Hit
ACCCATAACATCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCAT24110.40935245569027084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23270.39509048709716305No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGTATGATGTGAT22870.38829907348139747No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA22150.3760745289730194No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC21810.37030182739961864No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC20940.3555305027853285No Hit
GTCTTTGGGTATACATTTAAACCCTACCAAAACTAAAAGATGGGGTTACT20610.3499275865523219No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC19490.33091162842817823No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGCCCATGAA18200.30900931951733424No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG17970.305104256688269No Hit
GACCCATAACATCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCA16750.28439044516018397No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG16380.2781083875656008No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC16270.27624074882126526No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG16100.2733543980345649No Hit
CCCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT16080.27301482735377663No Hit
GGGTATACATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAA15690.26639319907840514No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG15620.2652047016956462No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA15500.2631672776109165No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG15410.26163920954736924No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT15300.2597715708030337No Hit
AACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGGTAG15110.256545649335545No Hit
GGTATACATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAA14890.252810371846874No Hit
TCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTT14850.25213123048529745No Hit
CTTTAAATTTCATGGGCTATGTTATTGGATGTTATGGGTCATTGCCACAA14690.2494146650389912No Hit
AGATCACATCATACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA14660.24890530901780877No Hit
AATTTAAAGAGTAACCCCATCTTTTAGTTTTGGTAGGGTTTAAATGTATA14590.2477168116350498No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT14460.245509602209926No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14200.2410951833596784No Hit
CATCATACATAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC13600.23090806293602997No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG13510.2293799948724827No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACATAAAATCAAAGAATGT13380.2271727854473589No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAATACG13370.22700300010696475No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC13110.22258858125671716No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA13100.222418795916323No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC13040.22140008387395815No Hit
ATAACATCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTT12970.22021158649119915No Hit
ACCACATTGTGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTT12840.21800437706607534No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG12750.21647630900252812No Hit
GTGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT12660.21494824093898085No Hit
ATGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGC12600.21392952889661604No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC12240.207817256642427No Hit
CAGTAAACCCTGTTCCGACTTCTGCCTCTCCCATATCGTCAATCTTCTCG12200.20713811528085044No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT12120.2057798325576973No Hit
GAGTAACCCCATCTTTTAGTTTTGGTAGGGTTTAAATGTATACCCAAAGA12030.20425176449415006No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACCAAAAC11850.20119562836705554No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA11560.19627185349562545No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11410.19372507338971337No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAACCCCAAAAGACCCACAAT11390.19338550270892513No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT11260.1911782932838013No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG10960.1860847330719771No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10870.18455666500842982No Hit
CCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGTATGATGT10800.18336816762567085No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCCT10700.18167031422172947No Hit
GGGTTACTCTTTAAATTTCATGGGCTATGTTATTGGATGTTATGGGTCAT10680.1813307435409412No Hit
GTATACATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAAT10610.1801422461581822No Hit
CCCATAACATCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATC10560.17929331945621152No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA10480.1779350367330584No Hit
GGCTATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA10170.17267169118084005No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGCAG9960.16910619903256313No Hit
CATTAAAGCAGGATAACCACATTGTGTGAAAGGGGCAGCAAACCCCAAAA9960.16910619903256313No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTATGTATGAT9950.168936413692169No Hit
GTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACATAAAAT9840.16706877494783345No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG9730.1652011362034979No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC9710.16486156552270964No Hit
TGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGCC9580.16265435609758583No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9570.1624845707571917No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA9530.16180542939561512No Hit
GATTTTATGTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATAA9480.16095650269364442No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC9250.15705143986457923No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC9120.1548442304394554No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT9100.15450465975866712No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC8970.1522974503335433No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8950.15195787965275503No Hit
CTTTCACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8910.15127873829117847No Hit
CCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCA8830.14992045556802533No Hit
ATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATG8830.14992045556802533No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT8810.14958088488723706No Hit
ACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGGTAGG8670.1472038901217191No Hit
CATCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGT8630.14652474876014254No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC8570.1455060367177777No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGGTTT8480.14397796865423046No Hit
CTCTTTAAATTTCATGGGCTATGTTATTGGATGTTATGGGTCATTGCCAC8400.14261968593107735No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC8390.1424499005906832No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA8380.14228011525028905No Hit
CTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTTATTGGATGT8370.14211032990989492No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAGGC8330.14143118854831838No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAG8330.14143118854831838No Hit
ATTTAAAGAGTAACCCCATCTTTTAGTTTTGGTAGGGTTTAAATGTATAC8300.14092183252713594No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8200.13922397912319454No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT8150.13837505242122386No Hit
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTTATTG8070.13701676969807072No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGGTAGGGTTTAAATG7980.13548870163452348No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAGGCAGGCTTTCACTTT7980.13548870163452348No Hit
CCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAG7920.1344699895921586No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTT7760.1317534241458524No Hit
TACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTA7650.12988578540151685No Hit
CTTTGATTTTATGTATGATGTGATCTTGTGGCAATGACCCATAACATCCA7620.12937642938033445No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG7540.12801814665718134No Hit
ATCTTGTGGCAATGACCCATAACATCCAATAACATAGCCCATGAAATTTA7390.12547136655126923No Hit
TTCTAAAACATTCTTTGATTTTATGTATGATGTGATCTTGTGGCAATGAC7260.12326415712614541No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7260.12326415712614541No Hit
AACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTTATTGGAT7190.12207565974338644No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG7160.12156630372220402No Hit
CCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGT7150.12139651838180987No Hit
TTCTTTGATTTTATGTATGATGTGATCTTGTGGCAATGACCCATAACATC7110.1207173770202333No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7040.11952887963747434No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTTCTGCCTCTCCCATATCGTCA7020.11918930895668606No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7010.11901952361629194No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC6970.11834038225471537No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGCCC6960.11817059691432122No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6940.11783102623353293No Hit
TTATGGGTCATTGCCACAAGATCACATCATACATAAAATCAAAGAATGTT6930.1176612408931388No Hit
TTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGG6930.1176612408931388No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG6770.11494467544683257No Hit
ACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAA6740.11443531942565016No Hit
CCTGTATTCAATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6660.11307703670249704No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6630.11256768068131462No Hit
ATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTAT6610.11222811000052633No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6570.11154896863894978No Hit
ATTGAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTG6540.11103961261776736No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG6470.10985111523500839No Hit
ATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACCAAAACTAAAAGA6440.10934175921382597No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6390.10849283251185528No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCCTAGATTGAA6370.108153261831067No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6330.10747412046949043No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT6240.10594605240594317No Hit
GATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGCCCATGA6210.10543669638476076No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC6210.10543669638476076No Hit
GGGCTATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATAC6200.10526691104436661No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCCTA6010.10204098957687796No Hit
GTTTTCTAAAACATTCTTTGATTTTATGTATGATGTGATCTTGTGGCAAT6010.10204098957687796No Hit
CCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTA5980.10153163355569553No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5910.10034313617293655No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5910.10034313617293655No Hit
GCAATGACCCATAACATCCAATAACATAGCCCATGAAATTTAAAGAGTAA5890.10000356549214827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)