FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548979
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23350.4253350310303309No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT23110.42096327910539383No Hit
GCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG23090.42059896644498246No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT22520.410216055623257No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCGTTTC17220.31367320061423115No Hit
CCTATTAGCAATATCTTCATGTAAATGAATTTTAGGAAACGAAGGGGTTT16580.30201519548106576No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA15860.2888999397062547No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA15230.277424090903295No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14230.2592084578827241No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT13550.24682182742873587No Hit
CATGAAGATATTGCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGT12440.22660247477590215No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG12040.2193162215676738No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA11790.21476231331253107No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11290.2056544968022456No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11250.20492587148142277No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT11050.2012827448773086No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTAAAACCCGACATTATT10570.19253924102743458No Hit
GTATGTAAATAATGTCGGGTTTTAAAGTAATGATTAACTACATGATCAGG10520.19162845937640602No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10180.1854351441494119No Hit
CTTTAATCCTGAATGGCAAACCCCTTCGTTTCCTAAAATTCATTTACATG10050.18306711185673769No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTTAAAGTAATGATTAACTACA9860.17960614158282923No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9560.17414145167665793No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT9340.17013401241213233No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT8780.15993325792061264No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG8720.1588403199393784No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8620.1570187566373213No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8490.15465072434464705No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA8430.15355778636341283No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT8220.14973250342909292No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG8200.1493681907686815No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC8150.14845740911765296No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA7840.14281056288127597No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT7800.14208193756045312No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7790.1418997812302474No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7740.14098899957921887No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7390.13461352802201906No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7310.1331562773803734No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT7230.13169902673872771No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT7080.12896668178564208No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7030.12805590013461354No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA6950.12659864949296787No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT6770.1233198355492651No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6660.12131611591700228No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG6650.12113395958679657No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT6630.12076964692638516No Hit
GTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCAT6570.1196767089451509No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCGTTTCCTAAAATTCA6470.11785514564309381No Hit
GTTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGCAT6420.11694436399206527No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6400.11658005133165386No Hit
ATCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA6350.11566926968062531No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6070.11056889243486545No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC6040.11002242344424831No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC6020.1096581107838369No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT5960.10856517280260267No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5930.10801870381198551No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5880.10710792216095698No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC5870.10692576583075128No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATA5870.10692576583075128No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCATTTTTGCCTTCT5870.10692576583075128No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5830.10619714050992843No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5790.1054685151891056No Hit
AATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGCCTTTAG5780.10528635885889989No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5780.10528635885889989No Hit
GATATTGCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGTAAATGA5760.10492204619848847No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5740.10455773353807704No Hit
GTTTTAAAGTAATGATTAACTACATGATCAGGATAATAAGGTTTAATCCC5640.10273617023601996No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC5610.10218970124540284No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG5610.10218970124540284No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5600.1020075449151971No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCGTTTCCT5580.10164323225478569No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC5580.10164323225478569No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5540.10091460693396287No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5530.10073245060375716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)