FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548979
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23170.4220562170866281No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT22480.4094874303024342No Hit
GCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG22470.40930527397222843No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT20320.370141662978001No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCGTTTC17130.31203379364237976No Hit
CCTATTAGCAATATCTTCATGTAAATGAATTTTAGGAAACGAAGGGGTTT16070.2927252226405746No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA15750.2868962200739919No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA15190.2766954655824722No Hit
CATGAAGATATTGCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGT13450.2450002641266788No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13060.23789616724865614No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT12790.23297794633310198No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA12160.22150209753014233No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG11860.21603740762397103No Hit
GTATGTAAATAATGTCGGGTTTTAAAGTAATGATTAACTACATGATCAGG11420.20802252909491986No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11280.2054723404720399No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTAAAACCCGACATTATT10780.19636452396175447No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT10440.19017120873476034No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9490.172866357365218No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9350.17031616874233804No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT9170.1670373547986353No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9130.16630872947781244No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTTAAAGTAATGATTAACTACA9090.16558010415698962No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9040.16466932250596106No Hit
CTTTAATCCTGAATGGCAAACCCCTTCGTTTCCTAAAATTCATTTACATG8980.1635763845247268No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG8880.16175482122266974No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8850.1612083522320526No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA8530.1553793496654699No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8490.15465072434464705No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG8200.1493681907686815No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA8010.14590722049477303No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT7930.14444996985312736No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT7590.13825665462613324No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC7570.13789234196572184No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7350.1338849027011962No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7270.13242765205955054No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT7240.13188118306893343No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7120.12969530710646493No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6860.12495924252111648No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT6850.12477708619091077No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6820.12423061720029363No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6810.12404846087008792No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT6740.12277336655864796No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCGTTTCCTAAAATTCA6630.12076964692638516No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA6550.11931239628473948No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6490.11821945830350525No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG6480.11803730197329952No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6460.1176729893128881No Hit
GTTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGCAT6430.11712652032227099No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT6410.11676220766185957No Hit
ATCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA6320.11512280069000819No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCATTTTTGCCTTCT6280.11439417536918534No Hit
GTTTTAAAGTAATGATTAACTACATGATCAGGATAATAAGGTTTAATCCC6260.11402986270877392No Hit
GATATTGCTAATAGGTGTCAACAATTTGTAGGCCCTCTCACTGTAAATGA6190.11275476839733396No Hit
GTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCAT6160.11220829940671684No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC6040.11002242344424831No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5950.10838301647239693No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC5940.10820086014219123No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC5760.10492204619848847No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5750.10473988986828275No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5720.10419342087766563No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC5700.10382910821725422No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCACTGCTCACCTCACCA5660.10310048289643137No Hit
ATCTTCATGTAAATGAATTTTAGGAAACGAAGGGGTTTGCCATTCAGGAT5640.10273617023601996No Hit
ATGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCAT5610.10218970124540284No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5590.1018253885849914No Hit
TGTTAATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATA5570.10146107592458No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5560.10127891959437428No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG5520.10055029427355146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)