FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689921
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG29730.43091890230910496No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG29620.42932451686497436No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG22940.33250183716686404No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG22660.32844340149089535No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA19460.2820612794798245No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA18650.2703208048457722No Hit
TGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGG17890.2593050508681429No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG17690.25640616824245094No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16740.24263647577041428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16630.24104209032628374No Hit
GATGAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC15880.23017128047993898No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT15490.22451845935983974No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG14870.2155319232201948No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA14580.21132854341294147No Hit
CCTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG14380.20842966078724956No Hit
GTGTAAAAGGAGCGGCAAAGCCCAGGAGACCCACAATTCTTTGACATACT14290.20712516360568817No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC13960.20234200727329651No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA13840.20060267769788134No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTTAGACAGGAAATGTGAAACCACA13610.19726896267833563No Hit
TTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAGAGGATATTATCA13110.19002175611410582No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC13040.18900714719511366No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC13040.18900714719511366No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTTAGA12930.18741276175098306No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT12670.18364421433758357No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC12210.17697678429849215No Hit
CCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATATAAAATACCAG12120.17567228711693078No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATGAACTGTATGTTCAGG11970.17349812514766183No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT11790.1708891307845391No Hit
GGAACATATTGTACAAAAAGTGAAACAATGTTTTCGGAAACTTCCTGTAA11470.16625091858343202No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT11230.1627722594326017No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCACTTTTTGT11120.16117787398847114No Hit
CCCATAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATATAAAATACCAG10910.1581340472314946No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA10780.15624977352479485No Hit
GTTTTCCAATCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGT10720.1553801087370873No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTAT10600.15364077916167215No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT10470.15175650545497238No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG10230.14827784630414206No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACATACAGTTAATCATTAC10180.14755312564771908No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9660.14001603082092007No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC9550.13842164537678953No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9470.13726209232651276No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCAC9450.13697220406394356No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA9410.13639242753880515No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9240.13392837730696702No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA9240.13392837730696702No Hit
CTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGC9220.13363848904439785No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9180.13305871251925946No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8990.13030477402485213No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8980.13015982989356753No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT8910.12914522097457534No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8680.12581150595502963No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTGTTCG8570.12421712051089909No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8470.1227676791980531No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCACTTTTTGTACAATATGTT8340.12088340549135336No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC8300.12030362896621498No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8050.11668002568410007No Hit
CAGTTCATCATTACTTCCAAACTAGACATTATTTACATACTCTGTGGAAG8040.11653508155281547No Hit
CTATCGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG7980.11566541676510789No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCACTTTTTGTACAAT7960.1153755285025387No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG7920.11479575197740031No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC7810.11320136653326975No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7610.11030248390757783No Hit
ATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA7520.10899798672601646No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA7450.10798337780702429No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7420.10754854541317048No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7250.10508449518133235No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7240.10493955105004776No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT7180.10406988626234018No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7150.1036350538684864No Hit
GCCCAGGAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7140.1034901097372018No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA7090.10276538908077881No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG7040.10204066842435583No Hit
CAGTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGTGGAAG7010.10160583603050205No Hit
CTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTAT7000.10146089189921745No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6980.10117100363664826No Hit
GTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCACTTTTTGTACAATA6970.10102605950536366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)