FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509854
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG15930.3124423854672122No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14040.27537294990330563No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG13970.2740000078453832No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12980.25458268445476545No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA12920.25340587697654626No Hit
ATCCTAACCTAACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT11360.22280888254284562No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC9790.19201575352944175No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC9700.19025054231211286No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA9610.188485331094784No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9560.18750465819626794No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9240.18122835164576526No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9100.17848246752992034No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9010.17671725631259144No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG8870.17397137219674652No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8860.1737752376170433No Hit
GGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTTAGGTT8800.17259843013882406No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA8750.171617757240308No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8480.16632212358832138No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7740.15180816469028388No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7690.15082749179176785No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7620.14945454973384537No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA7590.14886614599473574No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG7590.14886614599473574No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7310.14337437776304587No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7170.14062849364720095No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7120.13964782074868493No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7080.1388632824298721No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7060.13847101327046565No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6840.13415605251699506No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6820.13376378335758865No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTT6800.13337151419818222No Hit
GTACACCATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6760.13258697587936938No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6730.13199857214025976No Hit
GTCTAAGGGCAAATATTTGGTTAGGTTAGGATAGAACCTAGCAGGCATAA6440.1263106693288667No Hit
GTTCTATCCTAACCTAACCAAATATTTGCCCTTAGACAAAGGCATTAAAC6280.12317251605361536No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTCA6170.12101503567688005No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6100.11964209361895758No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6060.11885755530014475No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5900.1157194020248934No Hit
GTTGATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTGCC5810.11395419080756453No Hit
GTAGTAAGCTATGTCAACGTTAACATGGGCCTCAAAATCAGACAAATATT5790.11356192164815809No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA5650.11081603753231317No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGAAGTTTCCGAAAAC5630.11042376837290677No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5610.11003149921350033No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC5610.11003149921350033No Hit
CAATAGGTCTATTGACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT5400.10591267303973295No Hit
AGTCTGTACACCATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5350.10493200014121691No Hit
GGTCTATTGACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT5240.1027745197644816No Hit
CTTTATCTAAGGGCAAATATTTGGTTAGGTTAGGATAGAACCTAGCAGGC5120.1004209048080431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)