FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509854
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG20650.4050179070871269No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA13380.26242806764289384No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13100.256936299411204No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG12500.24516822462901144No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12000.235361495643851No Hit
ATCCTAACCTAACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT10660.20907946196362096No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9950.1951539068046931No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA9220.18083608248635885No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC9160.1796592750081396No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA9010.17671725631259144No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9010.17671725631259144No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG8950.1755404488343722No Hit
GGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTTAGGTT8900.17455977593585614No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC8800.17259843013882406No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8630.1692641422838695No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8470.16612598900861816No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8390.1645569123709925No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8350.16377237405217965No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA8140.15965354787841224No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7850.15396564506701918No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7760.15220043384969031No Hit
GTACACCATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7670.15043522263236142No Hit
GTCTAAGGGCAAATATTTGGTTAGGTTAGGATAGAACCTAGCAGGCATAA7620.14945454973384537No Hit
GTTCTATCCTAACCTAACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7500.14710093477740685No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7310.14337437776304587No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA7270.14258983944423304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7060.13847101327046565No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTT7020.13768647495165282No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG7000.1372942057922464No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT6900.1353328599952143No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6670.13082176466204049No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6660.1306256300823373No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6550.128468149705602No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6450.12650680390856991No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG6300.12356478521302176No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTCA5940.11650394034370623No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5860.11493486370608057No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5750.11277738332934525No Hit
GTTGATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTGCC5720.11218897959023565No Hit
GTAGTAAGCTATGTCAACGTTAACATGGGCCTCAAAATCAGACAAATATT5700.11179671043082921No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5660.11101217211201637No Hit
AGTCTGTACACCATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5650.11081603753231317No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5610.11003149921350033No Hit
CAATAGGTCTATTGACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT5500.10787401883676503No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTGTCTAAGGGC5470.1072856150976554No Hit
CTTTATCTAAGGGCAAATATTTGGTTAGGTTAGGATAGAACCTAGCAGGC5330.10453973098181048No Hit
GGTCTATTGACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT5220.10238225060507518No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5180.10159771228626234No Hit
GTCTGTACACCATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTT5150.1010093085471527No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA5130.1006170393877463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)