FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345104
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19900.5766377671658398No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA13430.38915805090639344No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT11060.32048310074644165No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9430.2732509620288377No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9420.27296119430664384No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT9340.27064305252909265No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7350.21297927581250867No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6890.1996499605915898No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT6870.19907042514720202No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6140.17791738142704808No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6050.17530947192730306No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6010.17415040103852752No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT5890.17067318837220086No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT5840.1692243497612314No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5710.16545736937271086No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG5370.1556052668181186No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTAAACAATACC5250.15212805415179192No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5210.15096898326301636No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5170.14980991237424082No Hit
GTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGTATGATGTGTT5170.14980991237424082No Hit
CACTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG5150.14923037692985303No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4950.14343502248597523No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4940.14314525476378134No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4920.14256571931939357No Hit
CTTTCCAATCAATGGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTG4910.1422759515971997No Hit
CTATGCATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4820.13966804209745468No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4770.13821920348648523No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTTACAGT4760.13792943576429134No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4710.13648059715332192No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT4650.13474199082015856No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4600.1332931522091891No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4570.13242384904260746No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4550.1318443135982197No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4520.13097501043163798No Hit
TATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGT4390.12720803004311743No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4370.12662849459872966No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4220.12228197876582132No Hit
CTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT4080.11822523065510686No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA4030.11677639204413742No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3900.11300941165561684No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT3850.11156057304464741No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC3820.11069126987806575No Hit
GACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCA3790.10982196671148407No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3790.10982196671148407No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA3720.10779359265612684No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3710.10750382493393296No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG3690.10692428948954517No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3640.10547545087857574No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA3630.10518568315638184No Hit
GAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3530.10228800593444294No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3530.10228800593444294No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAACC3470.10054939960127962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)