FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345104
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19690.5705526449997682No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA13870.40190783068292457No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT12620.3656868654086884No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11400.3303352033010339No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9040.26195002086327596No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT7930.2297858036997543No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7760.22485975242245815No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7540.21848486253419258No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7230.20950206314618203No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT7100.20573508275766147No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6760.19588298020306924No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT6410.1857411099262831No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6400.18545134220408918No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6210.1799457554824053No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6030.1747299364829153No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5990.17357086559413976No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5810.16835504659464973No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5100.14778153831888358No Hit
CTTTCCAATCAATGGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTG4960.1437247902081691No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4950.14343502248597523No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4930.14285548704158746No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4840.14024757754184247No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4720.1367703648755158No Hit
GTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTTTGTATGATGTGTT4670.13532152626454633No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTAAACAATACC4590.13300338448699522No Hit
CTATGCATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4530.1312647781538319No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT4530.1312647781538319No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4440.1286568686540869No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4380.12691826232092354No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT4350.1260489591543419No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4340.12575919143214798No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG4310.12488988826556632No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4270.12373081737679077No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4210.12199221104362742No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4120.11938430154388242No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4110.11909453382168854No Hit
CACTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG4100.11880476609949465No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTTACAGT4090.11851499837730077No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA3980.11532755343316796No Hit
GTTTGTAGGCCCACTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTA3930.11387871482219852No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3920.11358894710000464No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3830.11098103760025964No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC3820.11069126987806575No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3780.10953219898929017No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3760.1089526635449024No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3730.10808336037832075No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3610.10460614771199406No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3590.10402661226760629No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3480.1008391673234735No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG3460.10025963187908574No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC3460.10025963187908574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)