FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558923
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT26330.4710845679995277No Hit
GTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGG26190.4685797506991124No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA20200.3614093533456308No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG19110.3419075615066834No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA17260.30880819003691024No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15820.2830443549469247No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15810.2828654394254665No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG14210.2542389559921492No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA12540.22436006390862429No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12510.22382331734424957No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC12300.22006609139362665No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11960.21398296366404676No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC11890.21273055501383914No Hit
TCCACAGAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTA11770.21058356875634032No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG11630.20807875145592505No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11170.19984863746884632No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10880.1946600873465576No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT10880.1946600873465576No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT10870.19448117182509936No Hit
GAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCA10640.19036611483156No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10350.18517756470927121No Hit
ATTGACAACAGTGCCAGCATCGCCTCCTCCTGCTTCCACCAATCGGCAGT10330.18481973366635476No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA10120.18106250771573187No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTT9960.17819985937240013No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCT9930.17766311280802544No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTT9880.17676853520073427No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT9310.16657035047761498No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9120.16317095556990854No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8990.16084505379095154No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA8840.15816132096907803No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8700.15565650366866277No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTCCCAATTACATATCCCATGAA8670.15511975710428808No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8520.15243602428241457No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8490.15189927771803988No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8450.15118361563220695No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8430.15082578458929047No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA8290.14832096728887523No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8220.14706855863866758No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7950.14223783955929528No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7890.14116434643054587No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC7870.14080651538762942No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7850.14044868434471294No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7570.13543904974388243No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT7510.13436555661513302No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA7410.1325764014005507No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACCTTGCCACAG7360.13168182379325952No Hit
ATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC7220.12917700649284428No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAGACTAGACATTATT7170.1282824288855531No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7130.12756676679972018No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT7110.12720893575680373No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7000.12524086502076315No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7000.12524086502076315No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG6980.1248830339778467No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA6840.12237821667743143No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTGTACCTGTC6750.12076797698430732No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6740.12058906146284909No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6720.12023123041993262No Hit
CTACTACTTCCCTGGATGCTGGGTCTTCCAAATTACTCCCCACCCAGGTG6610.11826315968389205No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6510.11647400446930974No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6500.1162950889478515No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6410.1146848492547274No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCTGGA6290.1125378629972286No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6250.11182220091139566No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6240.11164328538993742No Hit
GTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC6230.11146436986847919No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC6190.11074870778264626No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6170.1103908767397298No Hit
ATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAG6130.10967521465389687No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGC6080.1087806370466057No Hit
GCTCTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6050.108243890482231No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTC6010.10752822839639807No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6010.10752822839639807No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5970.10681256631056514No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5960.1066336507891069No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5950.10645473526764866No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5910.10573907318181573No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5900.10556015766035752No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5870.1050234110959828No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA5850.10466558005306635No Hit
GCTTACTACTACTTCCCTGGATGCTGGGTCTTCCAAATTACTCCCCACCC5820.10412883348869165No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA5810.10394991796723341No Hit
ATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGGAT5800.10377100244577518No Hit
GATAAAGGCATTAAACCTTATTATCCTGAACATACAGTTAATCATTACTT5780.10341317140285872No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTG5730.10251859379556755No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCTGGAAG5690.10180293170973462No Hit
ATGTAATTGGGAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA5650.10108726962390169No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT5630.10072943858098521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)