FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558923
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGG28590.5115194758490883No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT21620.3868153573926999No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA19180.34315997015689104No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG17310.30970276764420146No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15620.27946604451776No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA15270.2732040012667219No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14370.2571016043354809No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA13090.2342004175888271No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG13020.23294800893861947No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG12670.2266859656875813No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC12410.22203416212966723No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA12140.21720344305029496No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11520.20611068071988448No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC10990.19662815808259815No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10930.19555466495384874No Hit
TCCACAGAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTA10650.1905450303530182No Hit
GAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCA10590.18947153722426882No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTT10400.18607214231656238No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10190.1823149163659395No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10180.18213600084448126No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT9990.17873660593677484No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9940.17784202832948368No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT9850.17623178863635958No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCT9350.1672860125634479No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9330.16692818152053146No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT9300.16639143495615674No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTT9290.1662125194346985No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9240.16531794182740733No Hit
ATTGACAACAGTGCCAGCATCGCCTCCTCCTGCTTCCACCAATCGGCAGT8970.16048722274803506No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTCCCAATTACATATCCCATGAA8800.1574456588832451No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8670.15511975710428808No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8660.15494084158282984No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8540.15279385532533102No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8490.15189927771803988No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8350.1493944604176246No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACCTTGCCACAG7980.14277458612366997No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7940.14205892403783704No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7880.14098543090908766No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7630.13651254287263184No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA7610.13615471182971536No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7580.13561796526534067No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC7150.12792459784263666No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA7140.12774568232117842No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTG7110.12720893575680373No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC7000.12524086502076315No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTGTACCTGTC6940.12416737189201374No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA6910.12363062532763905No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6850.12255713219888964No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAGACTAGACATTATT6780.12130472354868202No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6770.12112580802722378No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6730.12041014594139085No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6720.12023123041993262No Hit
ATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC6700.11987339937701616No Hit
GTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC6690.11969448385555792No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6610.11826315968389205No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6600.11808424416243384No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT6340.11343244060451976No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6310.11289569404014507No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCTGGA6260.1120011164328539No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA6170.1103908767397298No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT6080.1087806370466057No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG6030.10788605943931455No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6020.10770714391785631No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6020.10770714391785631No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5960.1066336507891069No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA5890.10538124213889928No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5850.10466558005306635No Hit
ATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGGAT5780.10341317140285872No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5780.10341317140285872No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5730.10251859379556755No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5710.1021607627526511No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5700.10198184723119286No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5670.10144510066681814No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5660.10126618514535993No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5650.10108726962390169No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5620.10055052305952697No Hit
ATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAG5620.10055052305952697No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC5600.10019269201661052No Hit
CTACTACTTCCCTGGATGCTGGGTCTTCCAAATTACTCCCCACCCAGGTG5590.10001377649515228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)